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Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis
Citation Link: https://doi.org/10.15480/882.3756
Publikationstyp
Journal Article
Date Issued
2021-06-03
Sprache
English
Author(s)
Edel, Miriam
Gescher, Johannes
TORE-DOI
Journal
Volume
14
Issue
1
Article Number
130
Citation
Biotechnology for Biofuels 14 (1): 130 (2021-12)
Publisher DOI
Scopus ID
Publisher
BioMed Central
Background: Some microorganisms can respire with extracellular electron acceptors using an extended electron transport chain to the cell surface. This process can be applied in bioelectrochemical systems in which the organisms produce an electrical current by respiring with an anode as electron acceptor. These organisms apply flavin molecules as cofactors to facilitate one-electron transfer catalyzed by the terminal reductases and in some cases as endogenous electron shuttles.
Results: In the model organism Shewanella oneidensis, riboflavin production and excretion trigger a specific biofilm formation response that is initiated at a specific threshold concentration, similar to canonical quorum-sensing molecules. Riboflavin-mediated messaging is based on the overexpression of the gene encoding the putrescine decarboxylase speC which leads to posttranscriptional overproduction of proteins involved in biofilm formation. Using a model of growth-dependent riboflavin production under batch and biofilm growth conditions, the number of cells necessary to produce the threshold concentration per time was deduced. Furthermore, our results indicate that specific retention of riboflavin in the biofilm matrix leads to localized concentrations, which by far exceed the necessary threshold value.
Conclusion: This study describes a new quorum-sensing mechanism in S. oneidensis. Biofilm formation of S. oneidensis is induced by low concentrations of riboflavin resulting in an upregulation of the ornithine-decarboxylase speC. The results can be applied for the development of strains catalyzing increased current densities in bioelectrochemical systems.
Results: In the model organism Shewanella oneidensis, riboflavin production and excretion trigger a specific biofilm formation response that is initiated at a specific threshold concentration, similar to canonical quorum-sensing molecules. Riboflavin-mediated messaging is based on the overexpression of the gene encoding the putrescine decarboxylase speC which leads to posttranscriptional overproduction of proteins involved in biofilm formation. Using a model of growth-dependent riboflavin production under batch and biofilm growth conditions, the number of cells necessary to produce the threshold concentration per time was deduced. Furthermore, our results indicate that specific retention of riboflavin in the biofilm matrix leads to localized concentrations, which by far exceed the necessary threshold value.
Conclusion: This study describes a new quorum-sensing mechanism in S. oneidensis. Biofilm formation of S. oneidensis is induced by low concentrations of riboflavin resulting in an upregulation of the ornithine-decarboxylase speC. The results can be applied for the development of strains catalyzing increased current densities in bioelectrochemical systems.
Subjects
Biofilm
Current density
Microbiology
Quorum sensing
Shewanella oneidensis
DDC Class
570: Biowissenschaften, Biologie
Funding Organisations
More Funding Information
This work was supported by a grant of the Bundesministerium für Bildung und Forschung (BMBF), No. 031B0847A. Proteomic experiments were partly supported by the ProFI Grant (ANR-10-INBS-08-01).
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