Publisher DOI: 10.1016/j.bioelechem.2019.04.022
Title: Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition
Language: English
Authors: Delgado, Veronica Palma 
Paquete, Catarina M. 
Sturm, Gunnar 
Gescher, Johannes 
Keywords: Bioelectrochemical system;Current density;Electron transfer;Genetic engineering;Periplasmic cytochrome;Shewanella oneidensis
Issue Date: Oct-2019
Source: Bioelectrochemistry 129: 18-25 (2019-10)
Journal: Bioelectrochemistry 
Abstract (english): 
Periplasmic c-type cytochromes are essential for the electron transport between the cytoplasmic membrane bound menquinol oxidase CymA and the terminal ferric iron reductase MtrABC in the outer membrane of Shewanella oneidensis cells. Either STC or FccA are necessary for periplasmic electron transfer. We followed the hypothesis that the elimination of potential competing reactions in the periplasm and the simultaneous overexpression of STC (cctA)could lead to an accelerated electron transfer to the cell surface. The genes nrfA, ccpA, napB and napA were replaced by cctA. This led to a 1.7-fold increased ferric iron reduction rate and a 23% higher current generation in a bioelectrochemical system. Moreover, the quadruple mutant had a higher periplasmic flavin content. Further deletion of fccA and its replacement by cctA resulted in a strain with ferric iron reduction rates similar to the wild type and a lower concentration of periplasmic flavin compared to the quadruple mutant. A transcriptomic analysis revealed that the quadruple mutant had a 3.7-fold higher cctA expression which could not be further increased by the replacement of fccA. This work indicates that a synthetic adaptation of Shewanella towards extracellular respiration holds potential for increased respiratory rates and consequently higher current densities.
ISSN: 1567-5394
Document Type: Article
Appears in Collections:Publications without fulltext

Show full item record

Page view(s)

Last Week
Last month
checked on Dec 2, 2021


Last Week
Last month
checked on Nov 26, 2021

PubMed Central

Last Week
Last month
checked on Dec 1, 2021

Google ScholarTM


Add Files to Item

Note about this record

Cite this record


Items in TORE are protected by copyright, with all rights reserved, unless otherwise indicated.