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An enzyme immobilized microreactor for continuous-flow biocatalysis of ginsenoside Rb1
Publikationstyp
Journal Article
Date Issued
2021-08-19
Sprache
English
Volume
96
Issue
12
Start Page
3349
End Page
3357
Citation
Journal of Chemical Technology and Biotechnology 96 (12): 3349-3357 (2021)
Publisher DOI
Scopus ID
Publisher
Wiley
BACKGROUND: Ginsenoside Rb1 is one of the major bioactive components of Panax ginseng C.A. Meyer (Araliaceae), a medicinal plant that has been used for therapeutic purposes for thousands of years in Asian countries. The pharmaceutical activity of ginsenoside Rb1 highly depends on molecular structure and its deglycosylated metabolites are known to be more potent bioactive compounds. However, these deglycosylated ginsenosides do not exist naturally so they are usually obtained by poorly selective methods, like chemical hydrolysis.
RESULTS: In this study, the development and characterization of an alginate-based immobilized enzyme microreactor for the catalytic conversion of ginsenoside Rb1 to more bioactive metabolites have been reported. Enzyme kinetic parameters were calculated and characterization tests (such as determination of surface area of alginate matrix, long-term use, and effect of residence time on conversion yield) were conducted. The system was operated under continuous-flow conditions and compared with acidic and batch enzymatic hydrolysis experiments, as conventional approaches. The enzymatic microreactor showed an enhanced activity by producing 13-fold higher amount of ginsenoside F2 than batch enzymatic hydrolysis.
CONCLUSION: Obtained results indicated that the newly developed enzymatic microreactor could successfully convert ginsenoside Rb1 to more active metabolites and have a potential for the biocatalysis of multiple ginsenosides, as well as pharmaceutically active compounds.
RESULTS: In this study, the development and characterization of an alginate-based immobilized enzyme microreactor for the catalytic conversion of ginsenoside Rb1 to more bioactive metabolites have been reported. Enzyme kinetic parameters were calculated and characterization tests (such as determination of surface area of alginate matrix, long-term use, and effect of residence time on conversion yield) were conducted. The system was operated under continuous-flow conditions and compared with acidic and batch enzymatic hydrolysis experiments, as conventional approaches. The enzymatic microreactor showed an enhanced activity by producing 13-fold higher amount of ginsenoside F2 than batch enzymatic hydrolysis.
CONCLUSION: Obtained results indicated that the newly developed enzymatic microreactor could successfully convert ginsenoside Rb1 to more active metabolites and have a potential for the biocatalysis of multiple ginsenosides, as well as pharmaceutically active compounds.
Subjects
alginate hydrogel
ginsenoside
immobilization
microreactor
β-glucosidase
DDC Class
540: Chemie
600: Technik
Funding Organisations
More Funding Information
This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, grant numbers:113 M050 and 2211-C) and the Federal Ministry of Education and Research (BMBF, grant number 01DL14002).