Dephenolization of palm oil mill effluent by oil palm fiber-immobilized Trametes hirsuta AK04 in temporary immersion bioreactor for the enhancement of biogas production

The dephenolization of palm oil mill effluent (POME) with oil palm fiber-immobilized Trametes hirsuta AK 04 was conducted in a temporary immersion bioreactor to reduce the inhibitory effects of phenolics in anaerobic digestion. Longer immersion times provided greater removal of phenolics due to a higher release of manganese peroxidase. The most effective dephenolization was observed at 6 h immersed and 2 h non-immersed time (immersion ratio 6/8) with maximum removal of 85% from 1277 mg L−1 of phenolics in 4 days. The immobilized fungus maintained its high activity during multiple repeated batch treatments. The pretreated POME of 2 h showed higher methane yields compared with the untreated POME substrate. The methane yields increased with increasing pretreatment time and dephenolization levels. The results suggested that an increased abundance of methanogens was associated with the detoxification of phenolics. The fungal biomass contained crude protein, amino acids, and essential phenolics, which can be used as animal feed supplements.

Subjects

Agro-industrial wastewater

Anaerobic digestion

Immobilized fungus

Phenolic compounds

Temporary immersion cultivation

White-rot fungi

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Dephenolization of palm oil mill effluent by oil palm fiber-immobilized Trametes hirsuta AK04 in temporary immersion bioreactor for the enhancement of biogas production