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Publisher DOI: 10.1002/aic.17158
Title: Multi-enzyme cascade reaction in a miniplant two-phase-system: model validation and mathematical optimization
Language: English
Authors: Johannsen, Jens  
Meyer, Francesca 
Engelmann, Claudia 
Liese, Andreas  
Fieg, Georg 
Bubenheim, Paul  
Waluga, Thomas 
Keywords: cascade reactions;enzymes;mathematical modeling;optimization;process integration;simulation
Issue Date: 11-Jan-2021
Publisher: Wiley
Source: AIChE Journal 67 (4): e17158 (2021-04)
Journal: AIChE journal 
Abstract (english): 
Biotechnological application of multiple enzymes in different phases for target compounds synthesis poses a significant challenge for industrial process development. At the same time, a growing demand for natural flavors and fragrances opens up possibilities for novel biotechnological processes to replace current chemical synthesis routes, with additional advantages such as avoiding harsh reaction conditions and toxic chemicals, and less by-products in the system. Within complex biotechnological processes, the key for unfolding their industrial application potential in bioprocess engineering lies in their mathematical modeling. In this contribution, a multi-enzyme cascade reaction in a two-phase system implemented in a miniplant-scale reactor setup is mathematically modeled for the example of the flavoring agent cinnamyl cinnamate. Using our validated model and a mathematical optimization tool based on a genetic algorithm, optimization runs are performed to demonstrate the potential of computer-aided process development for complex biotechnological processes.
DOI: 10.15480/882.3801
ISSN: 1547-5905
Institute: Systemverfahrenstechnik V-4 
Technische Biokatalyse V-6 
Document Type: Article
Project: Multienzymkaskade im 2-Phasensystem 
Projekt DEAL 
Funded by: Deutsche Forschungsgemeinschaft (DFG) 
License: CC BY 4.0 (Attribution) CC BY 4.0 (Attribution)
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