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Biocatalytic oxyfunctionalisation of Butane in bubble column reactors
Citation Link: https://doi.org/10.15480/882.4213
Publikationstyp
Doctoral Thesis
Date Issued
2022
Sprache
English
Author(s)
Perz, Sven Frederic
Advisor
Referee
Pörtner, Ralf
Title Granting Institution
Technische Universität Hamburg
Place of Title Granting Institution
Hamburg
Examination Date
2021-12-17
Institut
TORE-DOI
Citation
Technische Universität Hamburg (2022)
The selective oxyfunctionalisation of unactivated C-H bonds is considered to be a “dream reaction” in organic chemistry. In this study three biocatalytic systems for the oxyfunctionalisation of butane are investigated in a 0.2 L and 2 L bubble column reactor. (1) In a whole cell (E. coli) the heterologously expressed membrane bound alkane 1-monooxygenase (AlkBGT system) is applied to oxidise butane to butyric acid. (2) An improved strain is examined regarding its potential to metabolise butane and its derivatives, 1-butanol and butyric acid, as sole carbon source. (3) Unspecific peroxygenase (UPO) is applied as a free enzyme for the selective hydroxylation of butane to 2-butanol outside of analytical scale.
Subjects
Butane Oxidation
Bubble column reactor
AlkBGT Monooxygenase
UPO
Biotransformation
DDC Class
570: Biowissenschaften, Biologie
600: Technik
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