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Quantification of fructans in cereal-based bioethanol stillage based on a simplified analytical method
Citation Link: https://doi.org/10.15480/882.4350
Publikationstyp
Journal Article
Date Issued
2022-02-15
Sprache
English
Author(s)
TORE-DOI
Journal
Volume
99
Issue
4
Start Page
737
End Page
750
Citation
Cereal chemistry 99 (4): 737-750 (2022)
Publisher DOI
Scopus ID
Publisher
Wiley
Peer Reviewed
true
Background and Objectives
Cereal-based bioethanol stillage, the main by-product of industrial bioethanol production, is a potential substrate for fructans. However, the quantification of fructans in such complex sample matrices is still a challenge for the corresponding analytics to be overcome to allow for the identification and utilization of such unused fructan sources. Especially a possible utilization or rather the corresponding process development requires appropriate analytics first. Thus, this paper aims to develop and apply a method for fructan quantification in bioethanol stillage using common a high-performance liquid chromatography–refractive index detector.
Findings
A new approach for fructan quantification is presented allowing for simple fructan determination in bioethanol stillage for comparably high sample throughput. The developed analytical method performs fructan quantification by means of fructose- and glucose-equivalent determination after targeted acidic hydrolysis adapted to the respective sample matrix. On the basis of this method, up to 2.5% of fructans were found in the dry matter of cereal-based bioethanol stillage.
Conclusion
Cereal-based bioethanol stillage is assessed to be a potential source of fructans. The presented analytical method can be the basis for respective process development and, thus, may contribute to a higher-value utilization of stillage.
Significance and Novelty
According to current knowledge, no determination of fructans in stillage or corresponding analytical methods have been published so far.
Cereal-based bioethanol stillage, the main by-product of industrial bioethanol production, is a potential substrate for fructans. However, the quantification of fructans in such complex sample matrices is still a challenge for the corresponding analytics to be overcome to allow for the identification and utilization of such unused fructan sources. Especially a possible utilization or rather the corresponding process development requires appropriate analytics first. Thus, this paper aims to develop and apply a method for fructan quantification in bioethanol stillage using common a high-performance liquid chromatography–refractive index detector.
Findings
A new approach for fructan quantification is presented allowing for simple fructan determination in bioethanol stillage for comparably high sample throughput. The developed analytical method performs fructan quantification by means of fructose- and glucose-equivalent determination after targeted acidic hydrolysis adapted to the respective sample matrix. On the basis of this method, up to 2.5% of fructans were found in the dry matter of cereal-based bioethanol stillage.
Conclusion
Cereal-based bioethanol stillage is assessed to be a potential source of fructans. The presented analytical method can be the basis for respective process development and, thus, may contribute to a higher-value utilization of stillage.
Significance and Novelty
According to current knowledge, no determination of fructans in stillage or corresponding analytical methods have been published so far.
Subjects
Bioethanol stillage
Fructan quantification
Fructan analysis
Fructan hydrolysis
DDC Class
600: Technik
620: Ingenieurwissenschaften
Funding Organisations
More Funding Information
The project is supported by funds of the Federal Ministry of Food and Agriculture (BMEL) based on a decision of the Parliament of the Federal Republic of Germany via the Federal Office for Agriculture and Food (BLE) under the innovation support programme.
Publication version
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Cereal Chem - 2022 - Zimmermann - Quantification of fructans in cereal%u2010based bioethanol stillage based on a simplified.pdf
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