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Automatisierte Probenfiltration zur Analyse intrazellulärer Metaboliten bei höheren Biomassekonzentrationen
Citation Link: https://doi.org/10.15480/882.4441
Other Titles
Automated fast filtration for the analysis of intracellular metabolites at higher biomass concentrations
Publikationstyp
Journal Article
Publikationsdatum
2022-01-12
Sprache
German
Author
Enthalten in
Volume
94
Issue
6
Start Page
864
End Page
872
Citation
Chemie-Ingenieur-Technik 94 (6): 864-872 (2022-06-01)
Publisher DOI
Scopus ID
2-s2.0-85122656609
Publisher
Wiley-VCH Verl.
Studies of intracellular metabolites of Corynebacterium glutamicum mostly were done at levels of biomass concentrations below 10 g L−1. In this work we could show that it is also possible up to 32 g L−1 biomass concentration. Hereby we could point towards a faster filtration process with a modification of the already established automated fast filtration. We also could quantify the influence of a genetic modification of the lysine feedback inhibited asparto kinase in the aspartate metabolism with the automated fast filtration at higher biomass concentrations.
Schlagworte
Corynebacterium glutamicum
Fast filtration
Intracellular metabolites
Lysin biosynthesis
Intrazelluläre Metaboliten
Lysinbiosynthese
Schnellfiltration
DDC Class
600: Technik