Please use this identifier to cite or link to this item: https://doi.org/10.15480/882.4588
DC FieldValueLanguage
dc.contributor.authorArbter, Philipp-
dc.contributor.authorWidderich, Niklas-
dc.contributor.authorUtesch, Tyll-
dc.contributor.authorHong, Yaeseong-
dc.contributor.authorZeng, An-Ping-
dc.date.accessioned2022-09-16T05:08:44Z-
dc.date.available2022-09-16T05:08:44Z-
dc.date.issued2022-09-01-
dc.identifier.citationMicrobial cell factories 21 (1): 178 (2022-09-01)de_DE
dc.identifier.issn1475-2859de_DE
dc.identifier.urihttp://hdl.handle.net/11420/13615-
dc.description.abstractBACKGROUND: Electro-fermentation (EF) is an emerging tool for bioprocess intensification. Benefits are especially expected for bioprocesses in which the cells are enabled to exchange electrons with electrode surfaces directly. It has also been demonstrated that the use of electrical energy in BES can increase bioprocess performance by indirect secondary effects. In this case, the electricity is used to alter process parameters and indirectly activate desired pathways. In many bioprocesses, oxidation-reduction potential (ORP) is a crucial process parameter. While C. pasteurianum fermentation of glycerol has been shown to be significantly influenced electrochemically, the underlying mechanisms are not clear. To this end, we developed a system for the electrochemical control of ORP in continuous culture to quantitatively study the effects of ORP alteration on C. pasteurianum by metabolic flux analysis (MFA), targeted metabolomics, sensitivity and regulation analysis. RESULTS: In the ORP range of -462 mV to -250 mV, the developed algorithm enabled a stable anodic electrochemical control of ORP at desired set-points and a fixed dilution rate of 0.1 h-1. An overall increase of 57% in the molar yield for 1,3-propanediol was observed by an ORP increase from -462 to -250 mV. MFA suggests that C. pasteurianum possesses and uses cellular energy generation mechanisms in addition to substrate-level phosphorylation. The sensitivity analysis showed that ORP exerted its strongest impact on the reaction of pyruvate-ferredoxin-oxidoreductase. The regulation analysis revealed that this influence is mainly of a direct nature. Hence, the observed metabolic shifts are primarily caused by direct inhibition of the enzyme upon electrochemical production of oxygen. A similar effect was observed for the enzyme pyruvate-formate-lyase at elevated ORP levels. CONCLUSIONS: The results show that electrochemical ORP alteration is a suitable tool to steer the metabolism of C. pasteurianum and increase product yield for 1,3-propanediol in continuous culture. The approach might also be useful for application with further anaerobic or anoxic bioprocesses. However, to maximize the technique's efficiency, it is essential to understand the chemistry behind the ORP change and how the microbial system responds to it by transmitted or direct effects.en
dc.language.isoende_DE
dc.publisherBiomed Centralde_DE
dc.relation.ispartofMicrobial cell factoriesde_DE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/de_DE
dc.subjectBESde_DE
dc.subjectClostridium pasteurianumde_DE
dc.subjectContinuous fermentationde_DE
dc.subjectORPde_DE
dc.subjectRedox metabolismde_DE
dc.subjectRegulation analysisde_DE
dc.subjectSymbolic metabolic control analysisde_DE
dc.subject.ddc570: Biowissenschaften, Biologiede_DE
dc.subject.ddc600: Technikde_DE
dc.titleControl of redox potential in a novel continuous bioelectrochemical system led to remarkable metabolic and energetic responses of Clostridium pasteurianum grown on glycerolde_DE
dc.typeArticlede_DE
dc.identifier.doi10.15480/882.4588-
dc.type.diniarticle-
dcterms.DCMITypeText-
tuhh.identifier.urnurn:nbn:de:gbv:830-882.0196781-
tuhh.oai.showtruede_DE
tuhh.abstract.englishBACKGROUND: Electro-fermentation (EF) is an emerging tool for bioprocess intensification. Benefits are especially expected for bioprocesses in which the cells are enabled to exchange electrons with electrode surfaces directly. It has also been demonstrated that the use of electrical energy in BES can increase bioprocess performance by indirect secondary effects. In this case, the electricity is used to alter process parameters and indirectly activate desired pathways. In many bioprocesses, oxidation-reduction potential (ORP) is a crucial process parameter. While C. pasteurianum fermentation of glycerol has been shown to be significantly influenced electrochemically, the underlying mechanisms are not clear. To this end, we developed a system for the electrochemical control of ORP in continuous culture to quantitatively study the effects of ORP alteration on C. pasteurianum by metabolic flux analysis (MFA), targeted metabolomics, sensitivity and regulation analysis. RESULTS: In the ORP range of -462 mV to -250 mV, the developed algorithm enabled a stable anodic electrochemical control of ORP at desired set-points and a fixed dilution rate of 0.1 h-1. An overall increase of 57% in the molar yield for 1,3-propanediol was observed by an ORP increase from -462 to -250 mV. MFA suggests that C. pasteurianum possesses and uses cellular energy generation mechanisms in addition to substrate-level phosphorylation. The sensitivity analysis showed that ORP exerted its strongest impact on the reaction of pyruvate-ferredoxin-oxidoreductase. The regulation analysis revealed that this influence is mainly of a direct nature. Hence, the observed metabolic shifts are primarily caused by direct inhibition of the enzyme upon electrochemical production of oxygen. A similar effect was observed for the enzyme pyruvate-formate-lyase at elevated ORP levels. CONCLUSIONS: The results show that electrochemical ORP alteration is a suitable tool to steer the metabolism of C. pasteurianum and increase product yield for 1,3-propanediol in continuous culture. The approach might also be useful for application with further anaerobic or anoxic bioprocesses. However, to maximize the technique's efficiency, it is essential to understand the chemistry behind the ORP change and how the microbial system responds to it by transmitted or direct effects.de_DE
tuhh.publisher.doi10.1186/s12934-022-01902-5-
tuhh.publication.instituteBioprozess- und Biosystemtechnik V-1de_DE
tuhh.identifier.doi10.15480/882.4588-
tuhh.type.opus(wissenschaftlicher) Artikel-
dc.type.driverarticle-
dc.type.casraiJournal Article-
tuhh.container.issue1de_DE
tuhh.container.volume21de_DE
dc.relation.projectOpen-Access-Publikationskosten / 2022-2024 / Technische Universität Hamburg (TUHH)-
dc.identifier.pmid36050762de_DE
dc.rights.nationallicensefalsede_DE
dc.identifier.scopus2-s2.0-85137075183de_DE
tuhh.container.articlenumber178de_DE
local.status.inpressfalsede_DE
local.type.versionpublishedVersionde_DE
datacite.resourceTypeArticle-
datacite.resourceTypeGeneralJournalArticle-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.creatorOrcidArbter, Philipp-
item.creatorOrcidWidderich, Niklas-
item.creatorOrcidUtesch, Tyll-
item.creatorOrcidHong, Yaeseong-
item.creatorOrcidZeng, An-Ping-
item.languageiso639-1en-
item.creatorGNDArbter, Philipp-
item.creatorGNDWidderich, Niklas-
item.creatorGNDUtesch, Tyll-
item.creatorGNDHong, Yaeseong-
item.creatorGNDZeng, An-Ping-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.mappedtypeArticle-
crisitem.project.funderDeutsche Forschungsgemeinschaft (DFG)-
crisitem.project.funderid501100001659-
crisitem.project.funderrorid018mejw64-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptTechnische Biokatalyse V-6-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.orcid0000-0002-5166-0303-
crisitem.author.orcid0000-0003-4108-0345-
crisitem.author.orcid0000-0001-7514-7013-
crisitem.author.orcid0000-0002-5607-3618-
crisitem.author.orcid0000-0001-9768-7096-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik (V)-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik (V)-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik (V)-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik (V)-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik (V)-
Appears in Collections:Publications with fulltext
Files in This Item:
File Description SizeFormat
s12934-022-01902-5.pdfVerlags-PDF3,35 MBAdobe PDFView/Open
Thumbnail
Show simple item record

Page view(s)

56
checked on Dec 6, 2022

Download(s)

16
checked on Dec 6, 2022

Google ScholarTM

Check

Note about this record

Cite this record

Export

This item is licensed under a Creative Commons License Creative Commons