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Publisher DOI: 10.3389/fenvc.2022.931067
Title: Combining real-time fluorescence spectroscopy and flow cytometry to reveal new insights in DOC and cell characterization of drinking water
Language: English
Authors: Schuster, Jonas  
Huber, Johanna 
Stumme, Jakob 
Grieb, Anissa  
Ernst, Mathias  
Keywords: drinking water quality; real-time monitoring; dissolved organic matter; PARAFAC; bacterial growth; fluorescence spectroscopy; flow cytometry
Issue Date: 30-Aug-2022
Publisher: Frontiers Media S.A.
Source: Frontiers in Environmental Chemistry 3 : 931067 (2022-08-30)
Abstract (english): 
Sudden changes in drinking water quality can cause harmful consequences for end users. Thus, real-time monitoring of drinking water quality can allow early warning and provide crucial gains for securing safe water distribution. This study investigated the advantages of simultaneous real-time measuring of flow cytometry and fluorescence spectroscopy. A contamination event was investigated in a laboratory-scale analysis by spiking drinking water samples with organic nutrients. Flow cytometric data were analyzed by creating fingerprints based on differentiation into high and low nucleic acid cells (HNA/LNA). The detailed characterization of these data showed that an increase in HNA cells indicated an increase in the bacterial growth potential even before actual TCC increases. The fluorescence data was decomposed via the PARAFAC method to reveal seven fluorescent components. Three aromatic protein-like components were associated with the microbiological condition of the drinking water cells; namely, Components 4 (λ Ex = 279 nm, λ Em = 351 nm), 6 (λ Ex = 279 nm, λ Em = 332 nm), and 7 (λ Ex = 276 nm, λ Em = 302 nm). Component 6 was identified as a possible organic variable for appropriate monitoring of TCC, whereas Components 4 and 7 were identified as organic compounds representing nutrients for organisms present in drinking water. Overall, combining both methods for real-time monitoring can be a powerful tool to guarantee drinking water quality.
DOI: 10.15480/882.4595
ISSN: 2673-4486
Journal: Frontiers in Environmental Chemistry 
Institute: Wasserressourcen und Wasserversorgung B-11 
Document Type: Article
Project: Open-Access-Publikationskosten / 2022-2024 / Technische Universität Hamburg (TUHH) 
Online-Monitoring und digitale Steuerung in Trinkwasserversorgungssystemen 
Funded by: Deutsche Forschungsgemeinschaft (DFG) 
German-Israeli Water Technology Cooperation Program
License: CC BY 4.0 (Attribution) CC BY 4.0 (Attribution)
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