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Publisher DOI: 10.1186/s12934-017-0678-9
Title: Metabolic and proteomic analyses of product selectivity and redox regulation in Clostridium pasteurianum grown on glycerol under varied iron availability
Language: English
Authors: Groeger, Christin 
Wang, Wei 
Sabra, Wael 
Utesch, Tyll 
Zeng, An-Ping  
Keywords: n-Butanol; 1,3-Propanediol; C. pasteurianum; pasteurianum; product selectivity; metabolic analysis; proteomics
Issue Date: 19-Apr-2017
Publisher: BioMed Central
Source: Microbial cell factories (2017) 16:64
Abstract (english): 
Background: Clostridium pasteurianum as an emerging new microbial cell factory can produce both n-butanol (BuOH) and 1,3-propanediol (1,3-PDO), and the pattern of product formation changes significantly with the composition of the culture medium. Among others iron content in the medium was shown to strongly affect the products selectivity. However, the mechanism behind this metabolic regulation is still unclear. For a better understanding of such metabolic regulation and for process optimization, we carried out fermentation experiments under either iron excess or iron limitation conditions, and performed metabolic, stoichiometric and proteomic analyses.
Results: 1,3-PDO is most effectively produced under iron limited condition (Fe−), whereas 1,3-PDO and BuOH were both produced under iron rich condition (Fe+). With increased iron availability the BuOH/1,3-PDO ratio increased significantly from 0.27 mol/mol (at Fe−) to 1.4 mol/mol (at Fe+). Additionally, hydrogen production was enhanced significantly under Fe+ condition. Proteomic analysis revealed differentiated expression of many proteins including several ones of the central carbon metabolic pathway. Among others, pyruvate: ferredoxin oxidoreductase, hydrogenases, and several electron transfer flavoproteins was found to be strongly up-regulated under Fe+ condition, pointing to their strong involvement in the regeneration of the oxidized form of ferredoxin, and consequently their influences on the product selectivity in C. pasteurianum. Of particular significance is the finding that H2 formation in C. pasteurianum is coupled to the ferredoxin-dependent butyryl-CoA dehydrogenase catalyzed reaction, which significantly affects the redox balance and thus the product selectivity.
Conclusions: The metabolic, stoichiometric and proteomic results clearly show the key roles of hydrogenases and ferredoxins dependent reactions in determining the internal redox balance and hence product selectivity. Not only the NADH pool but also the regulation of the ferredoxin pool could explain such product variation under different iron conditions.
DOI: 10.15480/882.1404
ISSN: 1475-2859
Journal: Microbial cell factories 
Institute: Bioprozess- und Biosystemtechnik V-1 
Document Type: Article
Project: Open Access Publizieren 2016 - 2017 / Technische Universität Hamburg-Harburg 
License: CC BY 4.0 (Attribution) CC BY 4.0 (Attribution)
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