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  4. Engineering 'cell robots' for parallel and highly sensitive screening of biomolecules under in vivo conditions
 
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Engineering 'cell robots' for parallel and highly sensitive screening of biomolecules under in vivo conditions

Citation Link: https://doi.org/10.15480/882.1495
Publikationstyp
Journal Article
Date Issued
2017-11-09
Sprache
English
Author(s)
Song, Lifu  
Zeng, An-Ping  orcid-logo
Institut
Bioprozess- und Biosystemtechnik V-1  
TORE-DOI
10.15480/882.1495
TORE-URI
http://tubdok.tub.tuhh.de/handle/11420/1498
Journal
Scientific reports  
Volume
7
Issue
1
Start Page
15145
Citation
Scientific reports 1 (7): 15145- (2017-11-09)
Publisher DOI
10.1038/s41598-017-15621-0
Scopus ID
2-s2.0-85033698643
Publisher
Nature Publishing Group
Cells are capable of rapid replication and performing tasks adaptively and ultra-sensitively and can be considered as cheap "biological-robots". Here we propose to engineer cells for screening biomolecules in parallel and with high sensitivity. Specifically, we place the biomolecule variants (library) on the bacterial phage M13. We then design cells to screen the library based on cell-phage interactions mediated by a specific intracellular signal change caused by the biomolecule of interest. For proof of concept, we used intracellular lysine concentration in E. coli as a signal to successfully screen variants of functional aspartate kinase III (AK-III) under in vivo conditions, a key enzyme in L-lysine biosynthesis which is strictly inhibited by L-lysine. Comparative studies with flow cytometry method failed to distinguish the wild-type from lysine resistance variants of AK-III, confirming a higher sensitivity of the method. It opens up a new and effective way of in vivo high-throughput screening for functional molecules and can be easily implemented at low costs.
Subjects
High-throughput screening
Metabolic engineering
DDC Class
570: Biowissenschaften, Biologie
Funding(s)
Open Access Publizieren 2016 - 2017 / Technische Universität Hamburg-Harburg  
Lizenz
https://creativecommons.org/licenses/by/4.0/
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