Please use this identifier to cite or link to this item:
Publisher DOI: 10.3389/fmicb.2017.00448
Title: Improvement of the process stability of arylmalonate decarboxylase by immobilization for biocatalytic profen synthesis
Language: English
Authors: Aßmann, Miriam 
Mügge, Carolin 
Gaßmeyer, Sarah Katharina 
Enoki, Junichi 
Hilterhaus, Lutz 
Kourist, Robert 
Liese, Andreas  
Kara, Selin 
Keywords: arylmalonate decarboxylase;biocatalysis;enantioselectivity;immobilization;process stability;profen
Issue Date: 16-Mar-2017
Publisher: Frontiers Media
Source: Frontiers in microbiology (8): 448- (2017)
Journal or Series Name: Frontiers in microbiology 
Abstract (english): The enzyme arylmalonate decarboxylase (AMDase) enables the selective synthesis of enantiopure (S)-arylpropinates in a simple single-step decarboxylation of dicarboxylic acid precursors. However, the poor enzyme stability with a half-life time of about 1.2 h under process conditions is a serious limitation of the productivity, which results in a need for high catalyst loads. By immobilization on an amino C2 acrylate carrier the operational stability of the (S)-selective AMDase variant G74C/M159L/C188G/V43I/A125P/V156L was increased to a half-life of about 8.6 days, which represents a 158-fold improvement. Further optimization was achieved by simple immobilization of the cell lysate to eliminate the cost- and time intensive enzyme purification step.
DOI: 10.15480/882.1521
ISSN: 1664-302X
Institute: Technische Biokatalyse V-6 
Type: (wissenschaftlicher) Artikel
Project: Open Access Publizieren 2016 - 2017 / Technische Universität Hamburg-Harburg 
License: CC BY 4.0 (Attribution) CC BY 4.0 (Attribution)
Appears in Collections:Publications with fulltext

Files in This Item:
File Description SizeFormat
fmicb-08-00448.pdfVerlags-PDF1,99 MBAdobe PDFThumbnail
Show full item record

Page view(s)

Last Week
Last month
checked on Sep 27, 2020


checked on Sep 27, 2020

Google ScholarTM


Note about this record


This item is licensed under a Creative Commons License Creative Commons