TUHH Open Research
Help
  • Log In
    New user? Click here to register.Have you forgotten your password?
  • English
  • Deutsch
  • Communities & Collections
  • Publications
  • Research Data
  • People
  • Institutions
  • Projects
  • Statistics
  1. Home
  2. TUHH
  3. Publications
  4. Bioengineering of a full-thickness skin equivalent in a 96-well insert format for substance permeation studies and organ-on-a-chip applications
 
Options

Bioengineering of a full-thickness skin equivalent in a 96-well insert format for substance permeation studies and organ-on-a-chip applications

Citation Link: https://doi.org/10.15480/882.1698
Publikationstyp
Journal Article
Date Issued
2018-06-07
Sprache
English
Author(s)
Schimek, Katharina  
Hsu, Hao-Hsiang  
Boehme, Moritz  
Kornet, Jacob Jan  
Marx, Uwe  
Lauster, Roland  
Pörtner, Ralf 
Lindner, Gerd  
Institut
Bioprozess- und Biosystemtechnik V-1  
TORE-DOI
10.15480/882.1698
TORE-URI
http://tubdok.tub.tuhh.de/handle/11420/1701
Journal
Bioengineering  
Volume
5 (2018)
Issue
2
Start Page
Art.-Nr. 43
Citation
Bioengineering 5 (2): 43 (2018)
Publisher DOI
10.3390/bioengineering5020043
Scopus ID
2-s2.0-85056791350
Publisher
Multidisciplinary Digital Publishing Institute
The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attractive alternative to replace animal testing. The two-organ chip is designed to hold 96-well cell culture inserts (CCIs). Small-sized skin equivalents are needed for this. In this study, full-thickness skin equivalents (ftSEs) were generated successfully inside 96-well CCIs. These skin equivalents developed with in vivo-like histological architecture, with normal differentiation marker expressions and proliferation rates. The 96-well CCI-based ftSEs were successfully integrated into the two-organ chip. The permeation of fluorescein sodium salt through the ftSEs was monitored during the culture. The results show a decreasing value for the permeation over time, which seems a promising method to track the development of the ftSEs. Additionally, the permeation was implemented in a computational fluid dynamics simulation, as a tool to predict results in long-term experiments. The advantage of these ftSEs is the reduced need for cells and substances, which makes them more suitable for high throughput assays.
Subjects
full thickness skin equivalents
multi-organ chip
substance permeation
96-well cell culture insert
DDC Class
540: Chemie
570: Biowissenschaften, Biologie
610: Medizin
More Funding Information
Deutsche Forschungsgesellschaft (DFG) under grant No. PO413/12-1 and LA 1028/7-1
Lizenz
https://creativecommons.org/licenses/by/4.0/
Loading...
Thumbnail Image
Name

bioengineering-05-00043.pdf

Size

6.05 MB

Format

Adobe PDF

TUHH
Weiterführende Links
  • Contact
  • Send Feedback
  • Cookie settings
  • Privacy policy
  • Impress
DSpace Software

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science
Design by effective webwork GmbH

  • Deutsche NationalbibliothekDeutsche Nationalbibliothek
  • ORCiD Member OrganizationORCiD Member Organization
  • DataCiteDataCite
  • Re3DataRe3Data
  • OpenDOAROpenDOAR
  • OpenAireOpenAire
  • BASE Bielefeld Academic Search EngineBASE Bielefeld Academic Search Engine
Feedback