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Publisher DOI: 10.3844/ajbbsp.2013.243.254
Title: Influence of process conditions on measles virus stability
Language: English
Authors: Weiss, Katja 
Salzig, Denise 
Röder, Yasmin 
Gerstenberger, Jarrid 
Mühlebach, Michael D. 
Cichutek, Klaus 
Pörtner, Ralf  
Czermak, Peter 
Keywords: Measles Virus (MV);Virus Stability;Virus Inactivation;Oncolytic Agent
Issue Date: 2013
Publisher: Science Publications
Source: American Journal of Biochemistry and Biotechnology 3 (9): 243-254 (2013)
Journal or Series Name: American journal of biochemistry and biotechnology 
Abstract (english): Recombinant measles viruses are currently tested in clinical trials as oncolytic agent to be applied in cancer therapy. Contrary to their use as vaccine where 103infectious virus particles per dose are needed, for cancer therapy 109virus particles should be provided per dose. This leads to other challenges for the production process when compared to vaccine production. This study presents measles virus stability with regard to conditions during production and storage of the virus. Relevant process parameters such as temperature (4-37°C), pH (pH 4-11), conductivity (1.5 to 137.5 mS cm-1) and oxygen partial pressure were analyzed. The infectivity of measles virus particles decreased highly at 37 and 32°C, while at 22 and 4°C it remained stable for several hours or even days, respectively. The thermal inactivation reactions followed first order kinetics and the thermodynamic parameters enthalpy and entropy were estimated. Towards changes in pH measles virus particles were very sensitive, while no inactivation could be observed with varying conductivity. Measles virus incubation at an oxygen partial pressure of 100% did not lead to any loss of infectivity. The results show which parameters should be considered and controlled strongly in the production process to further raise measles virus yields for the high amount needed in cancer therapy approaches. © 2013 Science Publication.
DOI: 10.15480/882.2008
ISSN: 1558-6332
Institute: Bioprozess- und Biosystemtechnik V-1 
Type: (wissenschaftlicher) Artikel
Funded by: Deutsche Krebshilfe; Bundesministerium für Bildung und Forschung; Hessisches Ministerium für Wissenschaft und Kunst (LOEWE-Programm)
License: CC BY 3.0 (Attribution) CC BY 3.0 (Attribution)
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