Please use this identifier to cite or link to this item: https://doi.org/10.15480/882.2103
Publisher DOI: 10.1515/cdbme-2018-0029
Title: Bioimpedance analysis of L929 and HaCaT cells in low frequency range
Language: English
Authors: Teixeira, Viviane S. 
Kalckhoff, Jan-Patrick 
Krautschneider, Wolfgang 
Schröder, Dietmar 
Issue Date: 1-Sep-2018
Publisher: De Gruyter
Source: Current Directions in Biomedical Engineering 1 (4): 115-118 (2018-09-01)
Abstract (english): 
In this work, Bioimpedance Spectroscopy (BIS) is used to study fuids and cell solutions. A new four-electrode-terminal (4T) chamber using 3D printing and stainless steel corrosion resistant V4A was designed to measure the impedance of live cell solutions at the frequency range 0.1Hz-1MHz. At f < 1kHz the double layer (DL) that builds at electrode's surface raises the impedance substantially preventing the observation of the real impedance of the cells. The new 4T design circumvents the DL, is more robust and cheap, and allows for the repeatability of the results. Experiments were performed in vitro with two cell lines, L929 (mouse fbroblasts) and HaCaT (human keratinocytes). Results show that it is possible to distinguish between the two cell types by means of its BIS measurements in the new setup. Also, a low-frequency dispersion (α-dispersion) was observed in HaCaT cells solution, but not in L929. Furthermore, a potentiostat circuit model was developed in LTSpice to simulate the hardware setup and two different circuit models were used to ft cell's data. © 2018 Viviane S. Teixeira et al.
URI: http://hdl.handle.net/11420/2113
DOI: 10.15480/882.2103
ISSN: 2364-5504
Journal: Current directions in biomedical engineering 
Institute: Integrierte Schaltungen E-9 
Document Type: Article
License: CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives) CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives)
Appears in Collections:Publications with fulltext

Show full item record

Page view(s)

281
Last Week
1
Last month
3
checked on Mar 26, 2023

Download(s)

312
checked on Mar 26, 2023

SCOPUSTM   
Citations

5
Last Week
0
Last month
0
checked on Jun 30, 2022

Google ScholarTM

Check

Note about this record

Cite this record

Export

This item is licensed under a Creative Commons License Creative Commons