Publisher DOI: 10.1016/j.gene.2019.02.065
Title: Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes
Language: English
Authors: Bonitz, Martin 
Schaffer, Christiane 
Amling, Michael 
Pörtner, Ralf 
Schinke, Thorsten 
Jeschke, Anke 
Keywords: Articular chondrocyte;Matrix metalloproteinases;Osteoarthrtis;Syndecan-4;Synovial fibroblast
Issue Date: 25-Feb-2019
Source: Gene (698): 1-8 (2019-02-25)
Journal or Series Name: Gene 
Abstract (english): Although articular cartilage degeneration in osteoarthritis represents a major public health problem, there is still no molecular approach to prevent this pathology by blocking specific molecules. We have previously applied genome-wide expression analyses with porcine samples to identify specific markers of either growth plate or articular cartilage. Since the molecular differences were also found in cultured chondrocytes derived from both sites, we utilized primary porcine articular chondrocytes (PPACs) for the present study and analyzed, if and how they respond to synoviocyte-derived molecules. PPACs were treated by conditioned medium from porcine synovial fibroblasts (SF-CM) for 2, 6 and 24 h. Gene expression was subsequently monitored by qRT-PCR and microarray analysis. We found that short-term administration of SF-CM to PPACs significantly reduced expression of chondrocyte markers, while it induced expression of SDC4, encoding syndecan-4, a positive regulator of articular cartilage breakdown. Consistently, expression of MMP3, a putative downstream effector of syndecan-4 was strongly induced by SF-CM in PPACs. We identified an MMP3-inducing fraction in the range of 40 kDa after gel filtration, and we confirmed our findings in three-dimensional PPAC cultures, where SF-CM also reduced the glycosaminoglycan content. Taken together, our data suggest that synovial fibroblasts secrete one or more molecule(s) that activate specific signaling events in articular chondrocytes. Identifying a responsible ligand receptor pair(s) might pave the way to develop molecular therapies to reduce the severity of osteoarthritis.
URI: http://hdl.handle.net/11420/2196
ISSN: 0378-1119
Institute: Bioprozess- und Biosystemtechnik V-1 
Type: (wissenschaftlicher) Artikel
Funded by: This study was funded by the German Research Foundation (DFG; PO 413/14-1 , AM 103/21-1 ).
Appears in Collections:Publications without fulltext

Show full item record

Page view(s)

8
Last Week
0
Last month
3
checked on May 19, 2019

Google ScholarTM

Check

Export

Items in TORE are protected by copyright, with all rights reserved, unless otherwise indicated.