Please use this identifier to cite or link to this item: https://doi.org/10.15480/882.2186
DC FieldValueLanguage
dc.contributor.authorBlume, Grit-
dc.contributor.authorMüller-Wichards, Wiebke-
dc.contributor.authorGöpfert, Christiane-
dc.contributor.authorPörtner, Ralf-
dc.contributor.authorMüller, Jörg-
dc.date.accessioned2019-04-24T09:00:52Z-
dc.date.available2019-04-24T09:00:52Z-
dc.date.issued2013-
dc.identifier.citationThe open biomedical engineering journal 1 (7): 125-132 (2013)de_DE
dc.identifier.issn1874-1207de_DE
dc.identifier.urihttp://hdl.handle.net/11420/2344-
dc.description.abstractThe objective of this work involves the development and integration of electrodes for the electrical stimulation of cells within a bioreactor. Electrodes need to fit properties such as biocompatibility, large reversible charge transfer and high flexibility in view of their future application as implants on the tympanic membrane. Flexible thin-film platinum-poly(3,4-ethylene-dioxythiophene)-electrodes on a poly(ethylene terephthalate)-foil manufactured using microsystems technology were integrated into a bioreactor based on the design of a 24 well plate. The murine fibroblast cell line NIH-3T3 was cultured on the foil electrodes and the cells were stimulated with direct voltage and unipolar pulsed voltage. The amplitude, the pulse length and the ratio of pulse to pause were varied. The stimulated cells were stained in order to determine the angle between the cell cleavage plane of the dividing cells and the vector of the electric field. These angles were subsequently used to calculate the polarization index, which is a measure of the orientation of the metaphase plane of dividing cells that occurs for example during wound healing or embryonic morphogenesis.en
dc.description.sponsorshipBundesministerium für Bildung und Forschung (BMBF).de_DE
dc.language.isoende_DE
dc.publisherBentham Opende_DE
dc.relation.ispartofThe open biomedical engineering journalde_DE
dc.rightsCC BY-NC 3.0de_DE
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.subjectelectrical stimulationde_DE
dc.subjectcell culturede_DE
dc.subjectfibroblastsde_DE
dc.subjectintegrated biocompatible electrodesde_DE
dc.subjectorientation of metaphase platede_DE
dc.subjectpolarization index.de_DE
dc.subject.ddc570: Biowissenschaften, Biologiede_DE
dc.subject.ddc600: Technikde_DE
dc.subject.ddc610: Medizinde_DE
dc.subject.ddc620: Ingenieurwissenschaftende_DE
dc.titleElectrical stimulation of NIH-3T3 cells with platinum-PEDOT-electrodes integrated in a bioreactorde_DE
dc.typeArticlede_DE
dc.identifier.urnurn:nbn:de:gbv:830-882.030994-
dc.identifier.doi10.15480/882.2186-
dc.type.diniarticle-
dc.subject.ddccode600-
dc.subject.ddccode610-
dc.subject.ddccode570-
dc.subject.ddccode620-
dcterms.DCMITypeText-
tuhh.identifier.urnurn:nbn:de:gbv:830-882.030994-
tuhh.oai.showtruede_DE
tuhh.abstract.englishThe objective of this work involves the development and integration of electrodes for the electrical stimulation of cells within a bioreactor. Electrodes need to fit properties such as biocompatibility, large reversible charge transfer and high flexibility in view of their future application as implants on the tympanic membrane. Flexible thin-film platinum-poly(3,4-ethylene-dioxythiophene)-electrodes on a poly(ethylene terephthalate)-foil manufactured using microsystems technology were integrated into a bioreactor based on the design of a 24 well plate. The murine fibroblast cell line NIH-3T3 was cultured on the foil electrodes and the cells were stimulated with direct voltage and unipolar pulsed voltage. The amplitude, the pulse length and the ratio of pulse to pause were varied. The stimulated cells were stained in order to determine the angle between the cell cleavage plane of the dividing cells and the vector of the electric field. These angles were subsequently used to calculate the polarization index, which is a measure of the orientation of the metaphase plane of dividing cells that occurs for example during wound healing or embryonic morphogenesis.de_DE
tuhh.publisher.doi10.2174/1874120701307010125-
tuhh.publication.instituteBioprozess- und Biosystemtechnik V-1de_DE
tuhh.publication.instituteMikrosystemtechnik E-7de_DE
tuhh.identifier.doi10.15480/882.2186-
tuhh.type.opus(wissenschaftlicher) Artikel-
tuhh.institute.germanBioprozess- und Biosystemtechnik V-1de
tuhh.institute.englishBioprozess- und Biosystemtechnik V-1de_DE
tuhh.gvk.hasppnfalse-
openaire.rightsinfo:eu-repo/semantics/openAccessde_DE
dc.type.driverarticle-
dc.type.casraiJournal Article-
tuhh.container.issue1de_DE
tuhh.container.volume7de_DE
tuhh.container.startpage125de_DE
tuhh.container.endpage132de_DE
dc.relation.project01 EZ 0846de_DE
dc.relation.projectCharakterisierung der Zellwachstumsaktivität von Gewebezellen in 3D-Scaffolds mit integrierten Sensoren-
dc.rights.nationallicensefalsede_DE
item.creatorGNDBlume, Grit-
item.creatorGNDMüller-Wichards, Wiebke-
item.creatorGNDGöpfert, Christiane-
item.creatorGNDPörtner, Ralf-
item.creatorGNDMüller, Jörg-
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.grantfulltextopen-
item.creatorOrcidBlume, Grit-
item.creatorOrcidMüller-Wichards, Wiebke-
item.creatorOrcidGöpfert, Christiane-
item.creatorOrcidPörtner, Ralf-
item.creatorOrcidMüller, Jörg-
crisitem.author.deptMikrosystemtechnik E-7-
crisitem.author.deptMikrosystemtechnik E-7-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptMikrosystemtechnik E-7-
crisitem.author.orcid0000-0002-1332-2856-
crisitem.author.orcid0000-0003-1163-9718-
crisitem.author.parentorgStudiendekanat Elektrotechnik, Informatik und Mathematik-
crisitem.author.parentorgStudiendekanat Elektrotechnik, Informatik und Mathematik-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
crisitem.author.parentorgStudiendekanat Elektrotechnik, Informatik und Mathematik-
crisitem.project.funderDeutsche Forschungsgemeinschaft (DFG)-
crisitem.project.funderid501100001659-
crisitem.project.funderrorid018mejw64-
crisitem.project.grantnoPO 413/15-1 und TR 1203/1-1-
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