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Publisher DOI: 10.1016/j.btre.2019.e00333
Title: Optimization of solvent-free enzymatic esterification in eutectic substrate reaction mixture
Language: English
Authors: Pätzold, Magdalena 
Weimer, Anna 
Liese, Andreas  
Holtmann, Dirk 
Keywords: deep eutectic solvent;biocatalysis;water activity;reaction medium engineering;response surface methodology;design of experiments
Issue Date: 2019
Source: Biotechnology report (22): e00333- (2019))
Journal or Series Name: Biotechnology Reports 
Abstract (english): 
The Candida rugosa lipase catalyzed esterification of (-)-menthol and lauric acid (LA) was studied in a eutectic mixture formed by both substrates((-)-menthol:LA 3:1, mol/mol). No additional reaction solvent was necessary, since the (-)-menthol:LA deep eutectic solvent (DES) acts as combined reaction medium and substrate pool. Therefore, the esterification is conducted under solvent-free conditions. The thermodynamic water activity (aw) was identified as a key parameter affecting the esterification performance in the (-)-menthol:LA DES. A response surface methodology was applied to optimize the esterification conditions in terms aw, amount of C. rugosa lipase (mCRL) and reaction temperature. Under the optimized reaction conditions (aw = 0.55; mCRL =60 mg; T =45 °C), a conversion of 95 ± 1% LA was achieved (one day), the final (-)-menthyl lauric acid ester concentration reached 1.36 ± 0.04 M (2.25 days). The experimental product formation rate agreed very well with the model prediction.
DOI: 10.15480/882.2205
ISSN: 2215-017X
Institute: Technische Biokatalyse V-6 
Document Type: Article
Funded by: BMBF
Project: Niedrig schmelzende eutektische Solventien als Lösungsmittel für die Biokatalyse 
License: CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives) CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives)
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