Optimization of solvent-free enzymatic esterification in eutectic substrate reaction mixture

The Candida rugosa lipase catalyzed esterification of (-)-menthol and lauric acid (LA) was studied in a eutectic mixture formed by both substrates((-)-menthol:LA 3:1, mol/mol). No additional reaction solvent was necessary, since the (-)-menthol:LA deep eutectic solvent (DES) acts as combined reaction medium and substrate pool. Therefore, the esterification is conducted under solvent-free conditions. The thermodynamic water activity (aw) was identified as a key parameter affecting the esterification performance in the (-)-menthol:LA DES. A response surface methodology was applied to optimize the esterification conditions in terms aw, amount of C. rugosa lipase (mCRL) and reaction temperature. Under the optimized reaction conditions (aw = 0.55; mCRL =60 mg; T =45 °C), a conversion of 95 ± 1% LA was achieved (one day), the final (-)-menthyl lauric acid ester concentration reached 1.36 ± 0.04 M (2.25 days). The experimental product formation rate agreed very well with the model prediction.

Subjects

deep eutectic solvent

biocatalysis

water activity

reaction medium engineering

response surface methodology

design of experiments

DDC Class

500: Naturwissenschaften

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https://creativecommons.org/licenses/by-nc-nd/4.0/

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1-s2.0-S2215017X18303217-main.pdf

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987.21 KB

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Optimization of solvent-free enzymatic esterification in eutectic substrate reaction mixture