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  4. First Glycoside Hydrolase Family 2 Enzymes from Thermus antranikianii and Thermus brockianus with β-Glucosidase Activity
 
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First Glycoside Hydrolase Family 2 Enzymes from Thermus antranikianii and Thermus brockianus with β-Glucosidase Activity

Citation Link: https://doi.org/10.15480/882.2239
Publikationstyp
Journal Article
Date Issued
2015-06-03
Sprache
English
Author(s)
Schröder, Carola  
Blank, Saskia  
Antranikian, Garabed  
Institut
Technische Mikrobiologie V-7  
TORE-DOI
10.15480/882.2239
TORE-URI
http://hdl.handle.net/11420/2613
Journal
Frontiers in bioengineering and biotechnology  
Volume
3
Start Page
1
End Page
9
Citation
Frontiers in bioengineering and biotechnology (3): 1-9 (2015)
Publisher DOI
10.3389/fbioe.2015.00076
Scopus ID
2-s2.0-84987626725
Publisher
Frontiers Media
Two glycoside hydrolase encoding genes (tagh2 and tbgh2) were identified from different Thermus species using functional screening. Based on amino acid similarities, the enzymes were predicted to belong to glycoside hydrolase (GH) family 2. Surprisingly, both enzymes (TaGH2 and TbGH2) showed twofold higher activities for the hydrolysis of nitrophenol-linked β-D-glucopyranoside than of -galactopyranoside. Specific activities of 3,966 U/mg for TaGH2 and 660 U/mg for TbGH2 were observed. In accordance, K m values for both enzymes were significantly lower when β-D-glucopyranoside was used as substrate. Furthermore, TaGH2 was able to hydrolyze cellobiose. TaGH2 and TbGH2 exhibited highest activity at 95 and 90°C at pH 6.5. Both enzymes were extremely thermostable and showed thermal activation up to 250% relative activity at temperatures of 50 and 60°C. Especially, TaGH2 displayed high tolerance toward numerous metal ions (Cu(2+), Co(2+), Zn(2+)), which are known as glycoside hydrolase inhibitors. In this study, the first thermoactive GH family 2 enzymes with β-glucosidase activity have been identified and characterized. The hydrolysis of cellobiose is a unique property of TaGH2 when compared to other enzymes of GH family 2. Our work contributes to a broader knowledge of substrate specificities in GH family 2.
Subjects
β-glucosidase
glycoside hydrolase
thermostable
thermus antranikianii
thermus brockianus
cellobiose
truncated domains
DDC Class
570: Biowissenschaften, Biologie
More Funding Information
German Federal Ministry of Education and Research (BMBF), German Research Foundation (DFG)
Lizenz
https://creativecommons.org/licenses/by/4.0/
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