Publisher DOI: | 10.1007/s00449-019-02089-7 | Title: | Model-assisted Design of Experiments as a concept for knowledge-based bioprocess development | Language: | English | Authors: | Möller, Johannes Kuchemüller, Kim Beatrice Steinmetz, Tobias Koopmann, Kirsten Pörtner, Ralf ![]() |
Keywords: | Chinese hamster ovary; Feeding profile; Modeling; Response surface | Issue Date: | May-2019 | Source: | Bioprocess and biosystems engineering 5 (42): 867-882 (2019-05) | Abstract (english): | Design of Experiments methods offer systematic tools for bioprocess development in Quality by Design, but their major drawback is the user-defined choice of factor boundary values. This can lead to several iterative rounds of time-consuming and costly experiments. In this study, a model-assisted Design of Experiments concept is introduced for the knowledge-based reduction of boundary values. First, the parameters of a mathematical process model are estimated. Second, the investigated factor combinations are simulated instead of experimentally derived and a constraint-based evaluation and optimization of the experimental space can be performed. The concept is discussed for the optimization of an antibody-producing Chinese hamster ovary batch and bolus fed-batch process. The same optimal process strategies were found if comparing the model-assisted Design of Experiments (4 experiments each) and traditional Design of Experiments (16 experiments for batch and 29 experiments for fed-batch). This approach significantly reduces the number of experiments needed for knowledge-based bioprocess development. |
URI: | http://hdl.handle.net/11420/2682 | ISSN: | 1615-7591 | Journal: | Bioprocess and biosystems engineering | Institute: | Bioprozess- und Biosystemtechnik V-1 | Document Type: | Article | Project: | IBÖ-04: mDoE-Toolbox - Software zur modellgestützten Optimierung biotechnologischer Prozesse IBÖM04:mDoE-Toolbox2-Neue mDoE Software-Toolbox zur modellgestützten Optimierung biotechnologischer Prozesse Modellgestützte Bioprozessentwicklung zur Herstellung von natürlichen Milcholigosacchariden für medizinische Anwendungen |
More Funding information: | Bundesministerium für Bildung und Forschung 031B0305,031B0577A |
Appears in Collections: | Publications without fulltext |
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