Publisher DOI: 10.1007/s00449-019-02089-7
Title: Model-assisted Design of Experiments as a concept for knowledge-based bioprocess development
Language: English
Authors: Möller, Johannes 
Kuchemüller, Kim Beatrice 
Steinmetz, Tobias 
Koopmann, Kirsten 
Pörtner, Ralf  
Keywords: Chinese hamster ovary; Feeding profile; Modeling; Response surface
Issue Date: May-2019
Source: Bioprocess and biosystems engineering 5 (42): 867-882 (2019-05)
Abstract (english): 
Design of Experiments methods offer systematic tools for bioprocess development in Quality by Design, but their major drawback is the user-defined choice of factor boundary values. This can lead to several iterative rounds of time-consuming and costly experiments. In this study, a model-assisted Design of Experiments concept is introduced for the knowledge-based reduction of boundary values. First, the parameters of a mathematical process model are estimated. Second, the investigated factor combinations are simulated instead of experimentally derived and a constraint-based evaluation and optimization of the experimental space can be performed. The concept is discussed for the optimization of an antibody-producing Chinese hamster ovary batch and bolus fed-batch process. The same optimal process strategies were found if comparing the model-assisted Design of Experiments (4 experiments each) and traditional Design of Experiments (16 experiments for batch and 29 experiments for fed-batch). This approach significantly reduces the number of experiments needed for knowledge-based bioprocess development.
ISSN: 1615-7591
Journal: Bioprocess and biosystems engineering 
Institute: Bioprozess- und Biosystemtechnik V-1 
Document Type: Article
Project: IBÖ-04: mDoE-Toolbox - Software zur modellgestützten Optimierung biotechnologischer Prozesse 
IBÖM04:mDoE-Toolbox2-Neue mDoE Software-Toolbox zur modellgestützten Optimierung biotechnologischer Prozesse 
Modellgestützte Bioprozessentwicklung zur Herstellung von natürlichen Milcholigosacchariden für medizinische Anwendungen 
More Funding information: Bundesministerium für Bildung und Forschung 031B0305,031B0577A
Appears in Collections:Publications without fulltext

Show full item record

Page view(s)

Last Week
Last month
checked on Jun 9, 2023


Last Week
Last month
checked on Jun 30, 2022

Google ScholarTM


Add Files to Item

Note about this record

Cite this record


Items in TORE are protected by copyright, with all rights reserved, unless otherwise indicated.