Publisher DOI: 10.1016/j.carbpol.2017.01.004
Title: An injectable alginate-based hydrogel for microfluidic applications
Language: English
Authors: Akay, Seref 
Heils, Rene 
Trieu, Hoc Khiem 
Smirnova, Irina 
Yesil-Celiktas, Ozlem 
Keywords: Alginate;Chitosan;Hydrogel;Monolith;Microfabrication;Microfluidics
Issue Date: 9-Jan-2017
Publisher: Elsevier Science
Source: Carbohydrate Polymers (161): 228-234 (2017-04-01)
Journal or Series Name: Carbohydrate polymers 
Abstract (english): The objective of this study was to develop an injectable alginate based formulation for immobilizing enzymes into microfluidic systems. The gelation was induced upon lowering the pH by addition of D-glucono-δ-lactone (GDL) and release of Ca+ ions from solid CaCO3. The effects of GDL concentration on enzymatic activity and gelation time were investigated. The results indicated that increasing the GDL concentration increased both surface area and enzymatic activity. Also, chitosan was added to the formulation at different ratios to enhance the stability of enzyme during immobilization. For microfluidic application, 100 μl spiral coil single channel microchip was fabricated and alginate GDL mixture containing β-glucosidase was injected to the microchannel prior to gelation. Enzymatic conversion was performed by pumping substrate (pNPG) through the microchannel. The results indicated that the entire substrate was converted continuously during 24 h without any leakage or deactivation of immobilized enzyme.
ISSN: 0144-8617
Institute: Thermische Verfahrenstechnik V-8 
Mikrosystemtechnik E-7 
Type: (wissenschaftlicher) Artikel
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