Publisher DOI: 10.1002/elsc.201600010
Title: Glucose-mediated regulation of glycerol uptake in rhodosporidium toruloides: insights through transcriptomic analysis on dual substrate fermentation
Language: English
Authors: Bommareddy, Rajesh Reddy 
Sabra, Wael 
Zeng, An-Ping  
Keywords: Catabolite repressio;Glycerol metabolism;Lipid;Mixed substrate;Transcriptome
Issue Date: 9-May-2016
Publisher: Wiley-VCH
Source: Engineering in Life Sciences 3 (17): 282-291 (2017-03-01)
Journal or Series Name: Engineering in life sciences 
Abstract (english): Rhodosporidium toruloides is a robust oleaginous yeast that can accumulate lipids to more than 70% of its dry cell mass. Even though it is extensively studied for its fermentation of substrates like glucose and glycerol, limited information is available about its metabolism of mixture of glucose and glycerol. During growth on mixture of glucose and glycerol a typical diauxic growth and higher lipid yields were observed. To understand this phenomenon, RNA-seq analysis was implemented to study the gene expression profiles during growth on mixtures mainly to elucidate regulation of glycerol metabolism. Insights into lipid biosynthesis on mixed substrates are provided at a systems level. Among others, transcriptional profiles showed that glycerol might be produced intracellularly and glycerol kinase (GUT1) and glycerol 3–phosphate dehydrogenase (GUT2) enzymes were not downregulated in the presence of glucose. Transcriptional analysis also showed that the regulation of glycerol uptake in the presence of glucose at transcriptional level is different from that observed in Saccharomyces cerevisiae.
URI: http://hdl.handle.net/11420/3222
ISSN: 1618-2863
Institute: Bioprozess- und Biosystemtechnik V-1 
Type: (wissenschaftlicher) Artikel
Funded by: Financial support of this investigation from the BMBF under the project ‘Bio4Oil project number: 03SF0467’ is gratefully acknowledged.
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