Publisher DOI: 10.1002/elsc.201500191
Title: Conversion of biodiesel-derived glycerol into biotechnological products of industrial significance by yeast and fungal strains
Language: English
Authors: Papanikolaou, Seraphim 
Rontou, Maria 
Belka, Aikaterini 
Athenaki, Maria 
Gardeli, Chryssavgi 
Mallouchos, Athanasios 
Kalantzi, Ourania 
Koutinas, Apostolis A. 
Kookos, Ioannis K. 
Zeng, An-Ping  
Aggelis, George 
Keywords: Biodiesel-derived glycero;Citric acid;Ethanol;Mannitol;Single cell oil
Issue Date: 31-Mar-2016
Publisher: Wiley-VCH
Source: Engineering in Life Sciences 3 (17): 262-281 (2017-03-01)
Journal or Series Name: Engineering in life sciences 
Abstract (english): Oleochemical activities (e.g. biodiesel production, fat saponification) generate annually very high amounts of concentrated glycerol-containing waters (called crude glycerol) as the principal residues of these processes. Crude glycerol is an industrial residue the valorization of which attracts remarkable and constantly increasing interest. In the current investigation, biodiesel-derived glycerol was employed as substrate for yeast and fungal strains cultivated under nitrogen-limited conditions in shake flasks. Glucose was employed as reference substrate. Several yeasts (Candida diddensiae, Candida tropicalis, Pichia ciferrii, Williopsis saturnus, Candida boidinii, and Candida oleophila) rapidly assimilated glucose and converted it into ethanol, despite aerobic conditions imposed, and were Crabtree-positive. None of these yeasts produced ethanol during growth on glycerol or accumulated significant quantities of lipid during growth on glucose or glycerol. Only Rhodosporidium toruloides produced notable lipid quantities from glucose and to lesser extent from glycerol. Yarrowia lipolytica LFMB 20 produced citrate ≈58 g/L growing on high-glucose media, while on high-glycerol media ≈42 g/L citrate and ≈18 g/L mannitol. During growth on glucose/glycerol blends, glycerol was assimilated first and thereafter glucose was consumed. Fungi produced higher lipid quantities compared with yeasts. High lipid quantities were produced by Mortierella ramanniana, Mucor sp., and mainly Mortierella isabellina, with glycerol being more adequate for M. ramanniana and glucose for Mucor sp. and M. isabellina. M. isabellina ATHUM 2935 produced lipids of 8.5 g/L, 83.3% w/w in dry cell weight (DCW) and conversion yield per unit of glucose consumed ≈0.25 g/g. The respective values on glycerol were 5.4 g/L, 66.6% w/w in DCW and ≈0.22 g/g. Lipids of all microorganisms were analyzed with regards to their fatty acid composition, and M. isabellina presented the closest similitude with rapeseed oil. Crude lipids produced by this fungus and extracted with chloroform/methanol blend, were composed mostly of triacylglycerols, thus indicating that these solvents are adequate for triacylglycerol extraction.
ISSN: 1618-2863
Institute: Bioprozess- und Biosystemtechnik V-1 
Type: (wissenschaftlicher) Artikel
Funded by: Financially supported by the project entitled “New bioprocess for microbial oil from crude glycerol and cellulosic sugars” (Acronym: BIO4OIL, project code 2359) financed by the General Secretariat for Research and Technology, Ministry of National Education and Religious Affairs, Greece (project action: “Bilateral S&T cooperation between Greece and Germany 2013–2015”).
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