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  4. Conversion of biodiesel-derived glycerol into biotechnological products of industrial significance by yeast and fungal strains
 
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Conversion of biodiesel-derived glycerol into biotechnological products of industrial significance by yeast and fungal strains

Publikationstyp
Journal Article
Date Issued
2016-03-31
Sprache
English
Author(s)
Papanikolaou, Seraphim  
Rontou, Maria  
Belka, Aikaterini  
Athenaki, Maria  
Gardeli, Chryssavgi  
Mallouchos, Athanasios  
Kalantzi, Ourania  
Koutinas, Apostolis A.  
Kookos, Ioannis K.  
Zeng, An-Ping  orcid-logo
Aggelis, George  
Institut
Bioprozess- und Biosystemtechnik V-1  
TORE-URI
http://hdl.handle.net/11420/3225
Journal
Engineering in life sciences  
Volume
17
Issue
3
Start Page
262
End Page
281
Citation
Engineering in Life Sciences 3 (17): 262-281 (2017-03-01)
Publisher DOI
10.1002/elsc.201500191
Scopus ID
2-s2.0-85016627837
Publisher
Wiley-VCH
Oleochemical activities (e.g. biodiesel production, fat saponification) generate annually very high amounts of concentrated glycerol-containing waters (called crude glycerol) as the principal residues of these processes. Crude glycerol is an industrial residue the valorization of which attracts remarkable and constantly increasing interest. In the current investigation, biodiesel-derived glycerol was employed as substrate for yeast and fungal strains cultivated under nitrogen-limited conditions in shake flasks. Glucose was employed as reference substrate. Several yeasts (Candida diddensiae, Candida tropicalis, Pichia ciferrii, Williopsis saturnus, Candida boidinii, and Candida oleophila) rapidly assimilated glucose and converted it into ethanol, despite aerobic conditions imposed, and were Crabtree-positive. None of these yeasts produced ethanol during growth on glycerol or accumulated significant quantities of lipid during growth on glucose or glycerol. Only Rhodosporidium toruloides produced notable lipid quantities from glucose and to lesser extent from glycerol. Yarrowia lipolytica LFMB 20 produced citrate ≈58 g/L growing on high-glucose media, while on high-glycerol media ≈42 g/L citrate and ≈18 g/L mannitol. During growth on glucose/glycerol blends, glycerol was assimilated first and thereafter glucose was consumed. Fungi produced higher lipid quantities compared with yeasts. High lipid quantities were produced by Mortierella ramanniana, Mucor sp., and mainly Mortierella isabellina, with glycerol being more adequate for M. ramanniana and glucose for Mucor sp. and M. isabellina. M. isabellina ATHUM 2935 produced lipids of 8.5 g/L, 83.3% w/w in dry cell weight (DCW) and conversion yield per unit of glucose consumed ≈0.25 g/g. The respective values on glycerol were 5.4 g/L, 66.6% w/w in DCW and ≈0.22 g/g. Lipids of all microorganisms were analyzed with regards to their fatty acid composition, and M. isabellina presented the closest similitude with rapeseed oil. Crude lipids produced by this fungus and extracted with chloroform/methanol blend, were composed mostly of triacylglycerols, thus indicating that these solvents are adequate for triacylglycerol extraction.
Subjects
Biodiesel-derived glycero
Citric acid
Ethanol
Mannitol
Single cell oil
DDC Class
570: Biowissenschaften, Biologie
620: Ingenieurwissenschaften
More Funding Information
Financially supported by the project entitled “New bioprocess for microbial oil from crude glycerol and cellulosic sugars” (Acronym: BIO4OIL, project code 2359) financed by the General Secretariat for Research and Technology, Ministry of National Education and Religious Affairs, Greece (project action: “Bilateral S&T cooperation between Greece and Germany 2013–2015”).
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