CMOS based capacitive biosensor with integrated tethered bilayer lipid membrane for real-time measurements

Journal
Biomedical engineering : joint journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering  
Volume
57
Issue
SUPPL. 1 TRACK-E
Start Page
1010
End Page
1013
Citation
Biomedizinische Technik SUPPL. 1 TRACK-E (57): 1010-1013 (2012)
Publisher DOI
10.1515/bmt-2012-4016
Scopus ID
2-s2.0-84898013600
Publisher
De Gruyter
Conventional methods to investigate ionophore concentration require complex laboratory equipment. One approach to reduce the size of devices for the biosensor application is the implementation of the sensor circuits on CMOS microchips. Thus a new approach for the detection of changes in the ionophore concentration in tethered Bilayer Lipid Membranes (tBLMs) implemented in CMOS has been developed. In case of ion transport across the membrane by ionophores the ion concentration in the spacer region of the membrane is increased. This causes a permittivity increase and therefore a change of the capacitance of the spacer region which can be measured. The advantages of the capacitive biosensor principle are label-free measurements with short measurement times in the millisecond range. The test of the sensor chip with a circuit that mimics the membrane shows very good agreement between simulation and measurement. In addition, a Valinomycin concentration of 100 nM has been detected. An estimation of the detection limit gives a value of about 10 pM. © 2012 by Walter de Gruyter Berlin Boston.
DDC Class
620: Ingenieurwissenschaften