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Cometabolic degradation of trichloroethene by rhodococcus sp. strain L4 immobilized on plant materials rich in essential oils
Publikationstyp
Journal Article
Date Issued
2010
Sprache
English
Institut
TORE-URI
Volume
76
Issue
14
Start Page
4684
End Page
4690
Citation
Applied and Environmental Microbiology 14 (76): 4684-4690 (2010)
Publisher DOI
Scopus ID
Publisher
American Society for Microbiology
The cometabolic degradation of trichloroethene (TCE) by Rhodococcus sp. L4 was limited by the loss of enzyme activity during TCE transformation. This problem was overcome by repeated addition of inducing substrates, such as cumene, limonene, or cumin aldehyde, to the cells. Alternatively, Rhodococcus sp. L4 was immobilized on plant materials which contain those inducers In their essential oils. Cumin seeds were the most suitable immobilizing material, and the immobilized cells tolerated up to 68 μM TCE and degraded TCE continuously. The activity of Immobilized cells, which had been inactivated partially during TCE degradation, could be reactivated by incubation in mineral salts medium without TCE. These findings demonstrate that immobilization of Rhodococcus sp. L4 on plant materials rich in essential oils is a promising method for efficient cometabolic degradation of TCE.
DDC Class
570: Biowissenschaften, Biologie
600: Technik
620: Ingenieurwissenschaften
More Funding Information
National Center of Excellence for Environmental and Hazardous Waste Management (NCE-EHWM)
Faculty of Science, Chu-lalongkorn University
90th Anniversary of Chulalongkorn University Fund, Chulalongkorn University
International Foundation for Science, Stockholm, Sweden
Organization for the Prohibition of Chemical Weapons, The Hague, Netherlands