Publisher DOI: 10.1017/S1431927616012708
Title: Visualization of cellular components in a Mammalian cell with liquid-cell transmission electron microscopy
Language: English
Authors: Besztejan, Stephanie 
Keskin, Sercan 
Manz, Stephanie 
Kassier, Günther 
Bücker, Robert 
Venegas-Rojas, Daybith 
Trieu, Hoc Khiem 
Rentmeister, Andrea 
Miller, R. J. Dwayne 
Keywords: Gold nanoparticles;In situ imaging;Liquid-cell TEM;Mammalian cells;Nanofluidic cell
Issue Date: 31-Jan-2017
Publisher: Cambridge University Press
Source: Microscopy and Microanalysis 1 (23): 46-55 (2017-02-01)
Journal or Series Name: Microscopy and microanalysis 
Abstract (english): We present liquid-cell transmission electron microscopy (liquid-cell TEM) imaging of fixed and non-fixed prostate cancer cells (PC3 and LNCaP) with high resolution in a custom developed silicon nitride liquid cell. Fixed PC3 cells were imaged for 90–120 min without any discernable damage. High contrast on the cellular structures was obtained even at low electron doses (~2.5 e − /nm 2 per image). The images show distinct structures of cell compartments (nuclei and nucleoli) and cell boundaries without any further sample embedding, dehydration, or staining. Furthermore, we observed dynamics of vesicles trafficking from the cell membrane in consecutive still frames in a non-fixed cell. Our findings show that liquid-cell TEM, operated at low electron dose, is an excellent tool to investigate dynamic events in non-fixed cells with enough spatial resolution (few nm) and natural amplitude contrast to follow key intracellular processes.
ISSN: 1435-8115
Institute: Mikrosystemtechnik E-7 
Type: (wissenschaftlicher) Artikel
Funded by: This work was funded by the Max Planck Society and supported by the excellence cluster “The Hamburg Centre for Ultrafast Imaging”.
Project: Nanofluidikchip für in-liquid TEM 
Appears in Collections:Publications without fulltext

Show full item record

Page view(s)

Last Week
Last month
checked on Sep 27, 2020

Google ScholarTM


Add Files to Item

Note about this record


Items in TORE are protected by copyright, with all rights reserved, unless otherwise indicated.