Options
Parallel incorporation of different fluorinated amino acids : on the way to "teflon" proteins
Publikationstyp
Journal Article
Date Issued
2010-07-19
Sprache
English
Institut
TORE-URI
Journal
Volume
11
Issue
11
Start Page
1505
End Page
1507
Citation
ChemBioChem 11 (11): 1505-1507 (2010)
Publisher DOI
Scopus ID
Publisher
Wiley-VCH Verlag
(Chemical Equation Presented) Nonstick proteins? We report the successful residue-specific fluorination of 24 positions in a lipase by simultaneous incorporation of three different noncanonical amino acids. The enzyme produced in a polyauxotrophic E. coli strain structurally tolerates all fluorine atoms and retains its catalytic activity very well. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Subjects
Enzyme activity
Lipases
Noncanonical amino acids
Protein engineering
Sense-codon reassignment
DDC Class
570: Biowissenschaften, Biologie
Funding(s)
More Funding Information
Federal Ministry of Education and Research of Germany