Publisher DOI: 10.1007/978-1-0716-0191-4_13
Title: Efficient optimization of process strategies with model-assisted design of experiments
Language: English
Authors: Kuchemüller, Kim Beatrice 
Pörtner, Ralf  
Möller, Johannes 
Keywords: Batch; Computer-aided methods; DoE; Experimental space; Fed-batch; Response surface
Issue Date: 2020
Source: Methods in Molecular Biology (2095): 235-249 (2020)
Abstract (english): 
Conventional design of experiments (DoE) methods require expert knowledge about the investigated factors and their boundary values and mostly lead to multiple rounds of time-consuming and costly experiments. The combination of DoE with mathematical process modeling in model-assisted DoE (mDoE) can be used to increase the mechanistic understanding of the process. Furthermore, it is aimed to optimize the processes with respect to a target (e.g., amount of cells, product titer), which also provides new insights into the process. In this chapter, the workflow of mDoE is explained stepwise including corresponding protocols. Firstly, a mathematical process model is adapted to cultivation data of first experimental data or existing knowledge. Secondly, model-assisted simulations are treated in the same way as experimentally derived data and included as responses in statistical DoEs. The DoEs are then evaluated based on the simulated data, and a constrained-based optimization of the experimental space can be conducted. This loop can be repeated several times and significantly reduces the number of experiments in process development.
ISSN: 1064-3745
Journal: Methods in molecular biology 
Institute: Bioprozess- und Biosystemtechnik V-1 
Document Type: Chapter (Book)
Project: IBÖM04:mDoE-Toolbox2-Neue mDoE Software-Toolbox zur modellgestützten Optimierung biotechnologischer Prozesse 
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