|Publisher DOI:||10.1007/978-1-0716-0191-4_13||Title:||Efficient optimization of process strategies with model-assisted design of experiments||Language:||English||Authors:||Kuchemüller, Kim Beatrice
|Keywords:||Batch; Computer-aided methods; DoE; Experimental space; Fed-batch; Response surface||Issue Date:||2020||Source:||Methods in Molecular Biology (2095): 235-249 (2020)||Abstract (english):||
Conventional design of experiments (DoE) methods require expert knowledge about the investigated factors and their boundary values and mostly lead to multiple rounds of time-consuming and costly experiments. The combination of DoE with mathematical process modeling in model-assisted DoE (mDoE) can be used to increase the mechanistic understanding of the process. Furthermore, it is aimed to optimize the processes with respect to a target (e.g., amount of cells, product titer), which also provides new insights into the process. In this chapter, the workflow of mDoE is explained stepwise including corresponding protocols. Firstly, a mathematical process model is adapted to cultivation data of first experimental data or existing knowledge. Secondly, model-assisted simulations are treated in the same way as experimentally derived data and included as responses in statistical DoEs. The DoEs are then evaluated based on the simulated data, and a constrained-based optimization of the experimental space can be conducted. This loop can be repeated several times and significantly reduces the number of experiments in process development.
|URI:||http://hdl.handle.net/11420/4614||ISSN:||1064-3745||Journal:||Methods in molecular biology||Institute:||Bioprozess- und Biosystemtechnik V-1||Document Type:||Chapter (Book)||Project:||IBÖM04:mDoE-Toolbox2-Neue mDoE Software-Toolbox zur modellgestützten Optimierung biotechnologischer Prozesse|
|Appears in Collections:||Publications without fulltext|
Show full item record
checked on Jun 2, 2023
checked on Jun 30, 2022
Add Files to Item
Note about this record
Cite this record
Items in TORE are protected by copyright, with all rights reserved, unless otherwise indicated.