Publisher DOI: 10.1002/ceat.201600560
Title: Extended Elution by Characteristic Point Method for Characterization of Protein Ion-Exchange Adsorption
Language: English
Authors: Hartig, Dave 
Waluga, Thomas 
Schmidt, Claudia 
Fieg, Georg 
Scholl, Stephan 
Keywords: Adsorption isotherms;Elution by characteristic point;Ion-exchange adsorption;Protein adsorption
Issue Date: 1-Oct-2017
Source: Chemical Engineering and Technology 10 (40): 1940-1945 (2017-10-01)
Journal or Series Name: Chemical engineering & technology 
Abstract (english): An adequate characterization of adsorption isotherms is mandatory for chromatographic process development. Here, the elution by characteristic point (ECP) is a suitable method with low consumption of material and time. However, the ECP method requires a highly ideal behavior of the measured system. An innovative extended ECP approach is presented to characterize a non-ideal system by the example of protein ion-exchange adsorption. A marker, here bovine hemoglobin, is used to quantify all non-idealities, e.g., convection, dispersion, or low number of theoretical plates. The system signal of the marker is used to compensate the system signal of the interesting solute, here bovine serum albumin. The resulting isotherm agrees well with the control results of a validated static approach.
URI: http://hdl.handle.net/11420/4638
ISSN: 1521-4125
Institute: Prozess- und Anlagentechnik V-4 
Type: (wissenschaftlicher) Artikel
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