The geometrical structure of interfaces in dental enamel: A FIB-STEM investigation

Publikationstyp
Journal Article
Date Issued
2020-03-01
Sprache
English
Author(s)
Koldehoff, Jasmin  orcid-logo
Swain, Michael V.  
Schneider, Gerold A.  
Institut
Keramische Hochleistungswerkstoffe M-9  
TORE-URI
http://hdl.handle.net/11420/5145
Journal
Acta biomaterialia  
Volume
104
Start Page
17
End Page
27
Citation
Acta Biomaterialia (104): 17-27 (2020-03-01)
Publisher DOI
10.1016/j.actbio.2019.12.040
Scopus ID
2-s2.0-85077744845
In this study a high resolution structural analysis revealed that enamel prisms are surrounded by an interface that is discontinuous with frequent mineral to mineral contact separated by gaps. This contact manifests either by crystallites bridging the boundary between prismatic and interprismatic enamel or continuous crystallites curving and bridging the interprismatic enamel to the prisms. The geometrical resolution of this TEM investigation of the interfaces is ≤2 nm as a basis for micromechanical models. Within this resolution, contrary to existing structural descriptions of dental enamel structure in materials science literature, here the crystallites themselves are shown to be either in direct contact with each other, sometimes even fusing together, or are separated by gaps. Image analysis revealed that on average only 57 ± 15% of the interface consists of points of no contact between crystallites. This work reveals structural features of dental enamel that contribute important understanding to both the architecture and mechanical properties of this biological material. A new structural model is proposed and the implications for the mechanical properties of dental enamel are discussed. Statement of significance: In this study a high resolution structural analysis, employing focused ion beam and transmission electron microscopy revealed that enamel prisms are surrounded by interfaces that are discontinuous with frequent mineral to mineral contact separated by gaps. Although the interfaces in enamel have been investigated previously, existing studies are lacking in detail considering the geometry and morphology of the interfaces. We think that this result is of great importance when it comes to the understanding of the mechanical properties. In our opinion the concept of soft sheaths is no longer feasible. The resulting observations are included in a new structural model which provides new qualitative insights into the mechanical behavior. Existing analytical models were applied to simulate the new geometrical structure.
Subjects
Dental enamel
Hierarchy
Morphology
Protein sheaths
Transmission electron microscopy