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Publisher DOI: 10.1002/cctc.202000431
Title: Enzymatic Oxidation of Butane to 2-Butanol in a Bubble Column
Language: English
Authors: Perz, Frederic 
Ulber, Roland 
Alcalde, Miguel 
Bubenheim, Paul  
Hollmann, Frank 
Holtmann, Dirk 
Liese, Andreas  
Bormann, Sebastian 
Keywords: bubble column;butane;butanol;hydroxylation;peroxygenase
Issue Date: 9-Jun-2020
Publisher: Wiley
Source: ChemCatChem 14 (12): 3666-3669 (2020)
Journal or Series Name: ChemCatChem 
Abstract (english): Unspecific peroxygenases have recently gained significant interest due to their ability to catalyse the hydroxylation of non-activated C−H bonds using only hydrogen peroxide as a co-substrate. However, the development of preparative processes has so far mostly concentrated on benzylic hydroxylations using liquid substrates. Herein, we demonstrate the application of a peroxygenase for the hydroxylation of the inert, gaseous substrate butane to 2-butanol in a bubble column reactor. The influence of hydrogen peroxide feed rate and enzyme loading on product formation, overoxidation to butanone and catalytic efficiency is investigated at 200 mL scale. The process is scaled up to 2 L and coupled with continuous extraction. This setup allowed the production of 115 mmol 2-butanol and 70 mmol butanone with an overall total turnover number (TTN) of over 15.000, thereby demonstrating the applicability of peroxygenases for preparative hydroxylation of such inert, gaseous substrates at mild reaction conditions.
DOI: 10.15480/882.2850
ISSN: 1867-3899
Institute: Technische Biokatalyse V-6 
Type: (wissenschaftlicher) Artikel
Funded by: European Research Commision (ERC) grant, No 648026
License: CC BY 4.0 (Attribution) CC BY 4.0 (Attribution)
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