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  4. In vitro characterization of the effectiveness of enhanced sewage treatment processes to eliminate endocrine activity of hospital effluents
 
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In vitro characterization of the effectiveness of enhanced sewage treatment processes to eliminate endocrine activity of hospital effluents

Publikationstyp
Journal Article
Date Issued
2012-12-19
Sprache
English
Author(s)
Maletz, Sibylle  
Flöhr, Tilman  
Beier, Silvio  
Klümper, Claudia  
Brouwer, Abraham  
Behnisch, Peter  
Higley, Eric  
Giesy, John  
Hecker, Markus  
Gebhardt, Wilhelm  
Linnemann, Volker  
Pinnekamp, Johannes  
Hollert, Henner  
Institut
Abwasserwirtschaft und Gewässerschutz B-2  
TORE-URI
http://hdl.handle.net/11420/6909
Journal
Water research  
Volume
47
Issue
4
Start Page
1545
End Page
1557
Citation
Water Research 47 (4): 1545-1557 (2013)
Publisher DOI
10.1016/j.watres.2012.12.008
Scopus ID
2-s2.0-84873523288
Publisher
Elsevier Science
Occurrence of pharmaceuticals in aquatic ecosystems is related to sewage effluents. Due to the possible adverse effects on wildlife and humans, degradation and removal of pharmaceuticals and their metabolites during wastewater treatment is an increasingly important task.The present study was part of a proof of concept study at a medium sized country hospital in western Germany that investigated efficiency of advanced treatment processes to remove toxic potencies from sewage. Specifically, the efficiency of treatment processes such as a membrane bioreactor (MBR) and ozonation to remove endocrine disruptive potentials was assessed. Estrogenic effects were characterized by use of two receptor-mediated in vitro transactivation assays, the Lyticase Yeast Estrogen Screen (LYES) and the Estrogen Receptor mediated Chemical Activated LUciferase gene eXpression (ER CALUX®). In addition, the H295R Steroidogenesis Assay (H295R) was utilized to detect potential disruption of steroidogenesis. Raw sewage contained measurable estrogen receptor (ER)-mediated potency as determined by use of the LYES (28.9 ± 8.6 ng/L, 0.33× concentration), which was reduced after treatment by MBR (2.3 ± 0.3 ng/L) and ozone (1.2 ± 0.4 ng/L). Results were confirmed by use of ER CALUX® which measured concentrations of estrogen equivalents (EEQs) of 0.2 ± 0.11 ng/L (MBR) and 0.01 ± 0.02 ng/L (ozonation). In contrast, treatment with ozone resulted in greater production of estradiol and aromatase activity at 3× and greater concentrations in H295R cells. It is hypothesized that this is partly due to formation of active oxidized products during ozonation. Substance-specific analyses demonstrated efficient removal of most of the measured compounds by ozonation. A comparison of the ER-mediated responses measured by use of the LYES and ER CALUX® with those from the chemical analysis using a mass-balance approach revealed estrone (E1) to be the main compound that caused the estrogenic effects. Overall, treatment of sewage by use of MBR successfully reduced estrogenicity of hospital effluents as well as substances that are able to alter sex steroid production. However, after ozonation, effluents should undergo further investigations regarding the formation of endocrine active metabolites. The results obtained as part of this study demonstrated applicability of in vitro assays for monitoring of endocrine-modulating potency of treated sewage.
Subjects
Estrogenic action
Europe
H295R
LYES
Membrane bioreactor
Ozonation
DDC Class
540: Chemie
570: Biowissenschaften, Biologie
580: Pflanzen (Botanik)
590: Tiere (Zoologie)
620: Ingenieurwissenschaften
More Funding Information
* Landesamt für Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen (LANUV)
* RWTH Aachen University Undergraduate Research Opportunity Program (UROP)
* Canada Research Program
* Chair Professorship at the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong
* Einstein Professor Program of the Chinese Academy of Sciences
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