Ion-Exchange Adsorption of Proteins: Experiments and Molecular Dynamics Simulations

The adsorption of serum albumin and hemoglobin onto Q Sepharose FF was investigated, where the single and binary adsorption were considered. Both experiments and molecular dynamics simulations have been performed to study the adsorption from macroscopic and microscopic points of view. The steric mass-action model was applied to describe the single adsorption isotherms and to predict the binary isotherms. The results of the experiments and simulations were connected and compared by two parameters of the steric mass-action model. Both results present a preferential adsorption of serum albumin. The adsorption of serum albumin and hemoglobin onto Q Sepharose FF was studied. It combines experiments and molecular dynamics simulations. The single-component adsorption, simultaneous binary adsorption and sequential binary adsorption are investigated. A comprehensive understanding of ion-exchange adsorption of proteins is presented.

Subjects

Adsorption

Ion exchange

Protein

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Ion-Exchange Adsorption of Proteins: Experiments and Molecular Dynamics Simulations