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Publisher DOI: 10.3390/catal10101216
Title: A multi-enzyme cascade for the production of High-Value Aromatic Compounds
Language: English
Authors: Engelmann, Claudia 
Johannsen, Jens  
Waluga, Thomas 
Fieg, Georg 
Liese, Andreas  
Bubenheim, Paul  
Issue Date: 20-Oct-2020
Publisher: Multidisciplinary Digital Publishing Institute
Source: Catalysts 10 (10): 1216 (2020)
Journal: Catalysts 
Abstract (english): 
Cascade reactions are the basis of life in nature and are adapted to research and industry in an increasing manner. The focus of this study is the production of the high-value aromatic ester cinnamyl cinnamate, which can be applied in flavors and fragrances. A three-enzyme cascade was established to realize the synthesis, starting from the corresponding aldehyde with in situ cofactor regeneration in a two-phase system. After characterization of the enzymes, a screening with different organic solvents was carried out, whereby xylene was found to be the most suitable solvent for the second phase. The reaction stability of the formate dehydrogenase (FDH) from Candida boidinii is the limiting step during cofactor regeneration. However, the applied enzyme cascade showed an overall yield of 54%. After successful application on lab scale, the limitation by the FDH was overcome by immobilization of the enzymes and an optimized downstream process, transferring the cascade into a miniplant. The upscaling resulted in an increased yield for the esterification, as well as overall yields of 37%.
DOI: 10.15480/882.3028
ISSN: 2073-4344
Other Identifiers: doi: 10.3390/catal10101216
Institute: Technische Biokatalyse V-6 
Prozess- und Anlagentechnik V-4 
Document Type: Article
Project: Multienzymkaskade im 2-Phasensystem 
Publikationsfonds 2020 
More Funding information: Deutsche Forschungsgemeinschaft: BU3409/1-1 and WA 3957/1-1
License: CC BY 4.0 (Attribution) CC BY 4.0 (Attribution)
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