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  4. Metabolomic and kinetic investigations on the electricity-aided production of butanol by Clostridium pasteurianum strains
 
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Metabolomic and kinetic investigations on the electricity-aided production of butanol by Clostridium pasteurianum strains

Citation Link: https://doi.org/10.15480/882.3395
Publikationstyp
Journal Article
Publikationsdatum
2021-03
Sprache
English
Author
Arbter, Philipp 
Sabra, Wael 
Utesch, Tyll 
Hong, Yaeseong 
Zeng, An-Ping orcid-logo
Institut
Bioprozess- und Biosystemtechnik V-1 
DOI
10.15480/882.3395
TORE-URI
http://hdl.handle.net/11420/8207
Lizenz
https://creativecommons.org/licenses/by/4.0/
Enthalten in
Engineering in life sciences 
Volume
21
Issue
3-4
Start Page
181
End Page
195
Citation
Engineering in Life Sciences 21 (3-4): 181-195 (2021-03)
Publisher DOI
10.1002/elsc.202000035
Scopus ID
2-s2.0-85097162344
Publisher
Wiley-VCH
Engineering in Life Sciences published by Wiley-VCH GmbH In this contribution, we studied the effect of electro-fermentation on the butanol production of Clostridium pasteurianum strains by a targeted metabolomics approach. Two strains were examined: an electrocompetent wild type strain (R525) and a mutant strain (dhaB mutant) lacking formation of 1,3-propanediol (PDO). The dhaB-negative strain was able to grow on glycerol without formation of PDO, but displayed a high initial intracellular NADH/NAD ratio which was lowered subsequently by upregulation of the butanol production pathway. Both strains showed a 3–5 fold increase of the intracellular NADH/NAD ratio when exposed to cathodic current in a bioelectrochemical system (BES). This drove an activation of the butanol pathway and resulted in a higher molar butanol to PDO ratio for the R525 strain. Nonetheless, macroscopic electron balances suggest that no significant amount of electrons derived from the BES was harvested by the cells. Overall, this work points out that electro-fermentation can be used to trigger metabolic pathways and improve product formation, even when the used microbe cannot be considered electroactive. Accordingly, further studies are required to unveil the underlying (regulatory) mechanisms.
Schlagworte
BES
butanol production
Clostridium pasteurianum
electro-fermentation
redox metabolism
DDC Class
570: Biowissenschaften, Biologie
Projekt(e)
Publikationsfonds 2020 
Publikationsfonds 2021 
TUHH
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