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Projekt Titel
Development and microfluidic characterisation of a dynamically cultivated full skin model
Förderkennzeichen
PO 413/12-2
Funding code
945.03-744
Startdatum
April 16, 2016
Enddatum
April 15, 2019
Gepris ID
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Institut
Projektleitung
Pörtner, Ralf
Mitarbeitende
The proposal is based on the joint project -Development and characterization of a microfluidic dynamically cultured full-thickness skin model-. The aim of the project is the development of human skin models in dynamic culture conditions as well as their characterization with regard to substance transport, tissue formation and microfluidic. It is based on a multi-organ chip (MOC) as a new technology for modeling of segments of the human organism on a chip. This offers a unique platform for the development of physiologically approximated skin equivalents. By the adaptable construction method and quick design cycles, modular skin models can be developed stepwise including components like fatty tissue, hair follicle or a vascular system. In the current project this skin models were successfully developed and permeability coefficients for the simulation of mass transport and distribution effects with respect to the nutrients and the resulting metabolites in the supply and disposal of cell components as well as for large molecule ingredients were elaborated. Whereas so far the focus was on understanding the penetration of active ingredients, in the follow-up project penetration and permeation of industrially manufactured nanomaterials in human skin models will be studied. For this, new and meaningful study methods for the detection of penetration and permeation of nanomaterials in and through human skin will be established. The chip-based long term skin culture technology developed at TU Berlin will be optimized with respect to nanomaterial exposure and compared to other methods by in vitro and in vivo data.