Multienzymkaskade im 2-Phasensystem

Project Acronym
Project Title
Multienzymatic Cascade in a 2-Phase System
Funding Code
BU 3409/1-2 /// WA 3957/1-2
Principal Investigator
Project Abstract
Biocatalysis is of increasing importance as an alternative production process in chemical industry. In this context multienzymatic cascades have the potential to meet the demand for complex synthesis routes with high selectivity and purity of the products. Especially in-vitro systems have the potential for high space-time yields. They are less complex, comparatively simple to describe mathematically and therefor good to optimize. This project is about the biotechnological synthesis of cinnamyl cinnamate starting from cinnamaldehyde. Using a biomimetic Claisen-Tischtschenko reaction a sustainable production route of the ester is guaranteed. Isolated enzymes are used in a multiphasic reaction system. The aldehyde will be converted in the corresponding alcohol and acid by NAD(H) coupled sytnhesis. To ensure a high yield, these intermediates will be extracted to an organic phase. Within this phase the alcohol and acid are converted into the ester by a lipase. Removal of the ester will be carried out reaction integrated to ensure a continuous reaction system. The process will be realized in a recycle reactor with a stirred tank reactor for water phase reaction, a fixed bed reactor for organic phase reaction and a continuous operating centrifuge in-between. The present reaction system provides the innovative and novel use of two different in-situ product removal steps within one process. This provides a high potential for a continuous process with high space-time yield.This project aims to contribute to the development of a platform technology to combine process steps, which are usually individually performed. This research is about the continuous production of an in-vitro enzyme cascade with reaction integrated intermediate and reaction integrated product removal in a two phase system. The system will be described mathematical and modelled. The three enzymatic reactions and the mass transfer for the in-situ removal steps are described completely. In the end a whole process design will be given, based on the individual process steps, to give a valuable assistance for comparable processes.


Results 1-7 of 7

Issue DateTitleTypeAuthor(s)
120-Oct-2022Fulltext availableOpen AccessConcept of an enzymatic reactive extraction centrifugeArticleMeyer, Francesca ; Gasimov, Nijat ; Bubenheim, Paul  ; Waluga, Thomas 
2Sep-2022Fulltext availableOpen AccessCommissioning and operation of an enzymatic reactive extraction centrifugePosterMeyer, Francesca ; Liese, Andreas  ; Skiborowski, Mirko ; Bubenheim, Paul  ; Waluga, Thomas 
3Oct-2021Evaluation of process integration for the intensification of a biotechnological processArticleMeyer, Francesca ; Johannsen, Jens  ; Liese, Andreas  ; Fieg, Georg ; Bubenheim, Paul  ; Waluga, Thomas 
411-Jan-2021Fulltext availableOpen AccessMulti-enzyme cascade reaction in a miniplant two-phase-system: model validation and mathematical optimizationArticleJohannsen, Jens  ; Meyer, Francesca ; Engelmann, Claudia ; Liese, Andreas  ; Fieg, Georg ; Bubenheim, Paul  ; Waluga, Thomas 
520-Oct-2020Fulltext availableOpen AccessA multi-enzyme cascade for the production of High-Value Aromatic CompoundsArticleEngelmann, Claudia ; Johannsen, Jens  ; Waluga, Thomas ; Fieg, Georg ; Liese, Andreas  ; Bubenheim, Paul  
627-Jan-2020Fulltext availableOpen AccessEnzyme immobilization on synthesized nanoporous silica particles and their application in a bi-enzymatic reactionArticleEngelmann, Claudia ; Ekambaram, Narendhiran ; Johannsen, Jens  ; Fellechner, Oliver ; Waluga, Thomas ; Fieg, Georg ; Liese, Andreas  ; Bubenheim, Paul  
72020Pilot-scale operation of a multi-enzymatic cascade reaction in a multiphase systemArticleJohannsen, Jens  ; Engelmann, Claudia ; Liese, Andreas  ; Fieg, Georg ; Bubenheim, Paul  ; Waluga, Thomas