6 results
Filters
Settings
Now showing1 - 6 of 6
- Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Kyrpidia spormannii sp. nov., a thermophilic, hydrogenoxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus KyrpidiaA Gram-stain-positive, rod-shaped, non-motile, spore-forming bacterium, strain EA-1 T , was isolated from hydrothermal sediment samples from the Azores (S~ao Miguel, Portugal). 16S rRNA gene sequence analysis of the isolated bacterium revealed a phylogenetic affiliation with the genus Kyrpidia. The sequence similarity of the five 16S rRNA gene copies to its closest relative, Kyrpidia tusciae, ranged from 97.79 to 97.85 %. The in silico estimate of DNA-DNA hybridization was 56.0 %. The dominant fatty acids of the novel isolate were anteiso-C17: 0 (49.9 %), iso-C17: 0 (23.0 %) and iso-C16: 0 (13.3 %), while the quinone detected was menaquinone MK-7. Analysis of polar lipids identified phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and additional unidentified compounds comprising two glycolipids, two phospholipids and two lipids. The presence of meso-diaminopimelic acid in the peptidoglycan and mannose, arabinose and ribose in the cell wall of strain EA-1 T were detected. The strain was able to grow heterotrophically as well as autotrophically with carbon dioxide as the sole carbon source and with hydrogen and oxygen as electron donor and acceptor, respectively. Based on its chemotaxonomic, physiological and genomic characteristics, the new strain is considered to represent a novel species within the genus Kyrpidia, for which the name Kyrpidia spormannii sp. nov. is proposed. The type strain is strain EA-1 T (=DSM 106492 T =CCOS1194 T ).Publicationtype: Journal ArticleScopus© Citations 19 37 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Metallibacterium scheffleri gen. nov., sp. nov., an alkalinizing gammaproteobacterium isolated from an acidic biofilm(Microbiology Society, 2013-04-01); ; ; ; A Gram-stain-negative, non-motile, facultatively anaerobic, acid-tolerant rod, designated strain DKE6T, was isolated from an acidic biofilm (pH 2.5) harvested in the pyrite mine Drei Kronen und Ehrt in Germany. The isolate grew optimally at pH 5.5, between 25 and 30 °C and only with casein as the carbon and energy source; although a variety of sugars were tested as growth substrates, none supported growth of the isolate. During casein consumption, strain DKE6T produced and hence modulate the pH towards the growth optimum. The predominant fatty acids (>5%) were iso-C11:0 3-OH, iso-C15:0, iso-C17:0 and iso-C17:1 ω9c. The DNA G+C content was 66.6%. Strain DKE6T was not able to oxidize iron or thiosulfate. Iron reduction was detected. The isolate showed 93.3% 16S rRNA gene sequence similarity to the most closely related cultivable strain, Dokdonella koreensis DS-123T, but <93.2% sequence similarity with other type strains of closely related type species of the Gammaproteobacteria. On the basis of physiological and biochemical data, the isolate is considered to represent a novel species of a new genus in the class Gammaproteobacteria, for which we propose the name Metallibacterium scheffleri gen. nov., sp. nov. The type strain of the type species is DKE6T (=DSM 24874T=JCM 17596T).Publicationtype: Journal ArticleScopus© Citations 65 11 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Leucobacter chromiiresistens sp. nov., a chromate-resistant strain(Microbiology Society, 2011-04-01); ; ; ; A Gram-positive, irregular rod-shaped, non-motile, yellow-pigmented bacterium, strain JG 31T, was isolated in the course of identifying chromium-resistant soil bacteria. 16S rRNA gene sequence analysis of the isolated bacterium indicated its phylogenetic position within the genus Leucobacter. Binary 16S rRNA gene sequence alignments of the isolated bacterium with the 11 species of the genus recognized at the time of writing revealed sequence similarities of more than 97% with Leucobacter alluvii (GenBank accession no: AM072820; 99.4%), Leucobacter iarius (AM040493; 98.2%), Leucobacter aridicollis (AJ781047; 97.8%), Leucobacter komagatae (AB007419; 97.4%), Leucobacter chironomi (EU346911; 97.1%) and Leucobacter luti (AM072819; 97.1%). In contrast, DNA-DNA hybridization experiments showed similarity values below 28% for DNA samples from the most closely related type strains of L. alluvii, L. aridicollis and L. iarius. Protein analysis by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and automated RiboPrinting using the restriction enzyme PvuII differentiated strain JG 31T from all type strains of the genus Leucobacter. The dominant fatty acids of the novel isolate were anteiso-C15: 0, anteiso-C17: 0 and iso-C16: 0, while the quinone system consisted of menaquinones MK-11, MK-10, MK-9 and MK-8. In a B-type cross-linked peptidoglycan, the cell-wall amino acids were alanine, glycine, threonine, glutamic acid and 2,4-diaminobutyric acid. Strain JG 31T was able to grow in a medium containing up to 300 mM K2CrO4 and showed cellular aggregation in response to chromate stress. From biochemical and genomic analyses, the new strain is considered to represent a novel species of the genus Leucobacter, for which the name Leucobacter chromiiresistens sp. nov. is proposed. The type strain is strain JG 31T (=DSM 22788T =CCOS 200T).Publicationtype: Journal ArticleScopus© Citations 36 17 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Pseudoalteromonas arctica sp. nov., an aerobic, psychrotolerant, marine bacterium isolated from SpitzbergenA novel aerobic, psychrotolerant marine bacterium was isolated at 4 °C from seawater samples collected from Spitzbergen in the Arctic. The strain was a polar-flagellated, Gram-negative bacterium that grew optimally at 10-15 °C and pH 7-8 in media containing 2-3% NaCl (w/v), using various carbohydrates and organic acids as substrates. The main fatty acid components included 16 : 0 (12.7% of total fatty acids), straight-chain saturated fatty acid methyl ester (FAME) and 16 : 1ω7c (40.2 %) monounsaturated FAME. Phylogenetic analysis revealed a close relationship (99% 16S rRNA gene sequence similarity) between the novel isolate and Pseudoalteromonas elyakovii KMM 162T and some other species of the genus Pseudoalteromonas. The DNA G+C content of the novel strain was 39 mol%. DNA-DNA hybridization showed only 47.6% DNA-DNA relatedness with P. elyakovii KMM 162T, 44.2% with Pseudoalteromonas distincta KMM 638T and 22.6% with Pseudoalteromonas nigrifaciens NCIMB 8614T. Based on phylogenetic and phenotypic characteristics, this isolate represents a novel species of the genus Pseudoalteromonas for which the name Pseudoalteromonas arctica is proposed; the type strain is A 37-1-2T (=LMG 23753T=DSM 18437T). © 2008 IUMS.Publicationtype: Journal Article150 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Spongiispira norvegica gen. nov., sp. nov., a marine bacterium isolated from the boreal sponge Isops phlegraei(SGM, 2008-08-01); ; ; ; The bacterial strain Gp₄₇.1T, isolated from the marine sponge Isops phlegraei collected at the Sula Ridge off the Norwegian coast, was characterized. The isolate was a motile spirillum that was monopolarly and monotrichously flagellated. It was aerobic, Gram-negative, oxidase-positive and catalase-negative. Optimal growth occurred between 20 and 30 °C, at pH 7-8 and with a salt concentration of 2-3% (w/v). The isolate showed a relatively restricted nutritional profile. Substrate utilization tests were only positive for arabinose. Enzyme tests were positive for esterase lipase C8, lipase C14, leucine arylamidase and naphthol-AS-BI-phosphohydrolase. The strain was not able to reduce nitrate. The major cellular fatty acids were C16:1ω7 and C16:0. The DNA G+C content was 62.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison classified the strain as a member of the order Oceanospirillales in the class Gammaproteobacteria. Strain Gp₄₇.1T formed a distinct phyletic line with less than 94% 16S rRNA gene sequence similarity to its closest relatives with validly published names. Based on the determined data, it is proposed that the strain represents a novel species in a new genus, Spongiispira norvegica gen. nov., sp. nov.; the type strain of Spongiispira norvegica is Gp₄₇.1T (=DSM 17749T =NCIMB 14401T). © 2008 IUMS.Publicationtype: Journal Article75 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Spongiibacter marinus gen. nov., sp. nov., a halophilic marine bacterium isolated from the boreal sponge Haliclona sp. 1(SGM, 2008-03-01); ; ; ; Strain HAL40bT was isolated from the marine sponge Haliclona sp. 1 collected at the Sula Ridge off the Norwegian coast and characterized by physiological, biochemical and phylogenetic analyses. The isolate was a small rod with a polar flagellum. It was aerobic, Gram-negative and oxidase- and catalase-positive. Optimal growth was observed at 20-30 °C, pH 7-9 and in 3% NaCl. Substrate utilization tests were positive for arabinose, Tween 40 and Tween 80. Enzyme tests were positive for alkaline phosphatase, esterase lipase (C8), leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase and N-acetyl-β-glucosaminidase. The predominant cellular fatty acid was C17:1ω8, followed by C17:0 and C18:1ω7. Analysis by matrix-assisted laser desorption/ ionization time-of-flight MS was used to characterize the strain, producing a characteristic low-molecular-mass protein pattern that could be used as a fingerprint for identification of members of this species. The DNA G+C content was 69.1 mol%. Phylogenetic analysis supported by 16S rRNA gene sequence comparison classified the strain as a member of the class Gammaproteobacteria. Strain HAL40bT was only distantly related to other marine bacteria including Neptunomonas naphthovorans and Marinobacter daepoensis (type strain sequence similarity >90%). Based on its phenotypic, physiological and phylogenetic characteristics, it is proposed that the strain should be placed into a new genus as a representative of a novel species, Spongiibacter marinus gen. nov., sp. nov.; the type strain of Spongiibacter marinus is HAL40bT (=DSM 17750T =CCUG 54896T). © 2008 IUMS.Publicationtype: Journal Article84