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    Item type:Publication,
    STUDY OF CHEMICAL COMPOSITION AND PROPERTIES OF BIOMASS OF CHLORELLA SOROKINIANA UNDER INFLUENCE OF DIFFERENT PHYSICAL FACTORS
    The paper presents data on the current issue of the processing of microalgae Chlorella sorokiniana, the richness of the vitamin and amino acid composition of which ensures its nutri-tional (twice as much as soy protein) and biological value. This article also provides information on the utilization of chlorella as an enrichment supplement to develop a modern Life Support Sys-tem for manned spacecraft. Based on the data collected from various bibliographical studies, it is recommended to take into account the established positive effect of light exposure on the microal-gae for the production of astaxanthin, as well as, to vary the composition of the nutrient medium in order to obtain a dry microalgae biomass with a fixed lipid, protein and starch content. The influence of various physical factors on the specificity of biochemical processes in the cell and the variation in the composition of dry biomass in the cultivation of Chlorella sorokiniana was studied. Experimental results of the application of a red laser (output power 1.6 mW, wavelength 0.63 μm), the exposure to daylight (intensity 2800 Lux), infrared (power 250 W, intensity 14100 Lux) and ultraviolet (wavelength 280-315 nm) radiations of the microalgal samples are presented. The most significant concentrations of protein compounds (46.0% and 48.4% of dry biomass) were obtained after the exposure to daylight and to infrared radiation. In the last case, the fatty acid composition of the sample was represented mainly by unsaturated acids (77% by weight). The biomass of C. sorokiniana cultivated under the influence of daylight showed an increased content of chlorophylls a (19.3 mg/g), chlorophyll b (7.1 mg/g) and of total carotenoids (2.8 mg/g). Under the action of ultraviolet light, the quantity of saccharides increased (up to 430 mg/g). The data obtained enables to select optimal cultivation conditions of Chlorella sorokiniana in order to obtain a biomass with a given composition and properties.
    Publicationtype: Journal Article
      180
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    Item type:Publication,
    Waste-free technology of Сhlorella sorokiniana microalgae biomass usage for lipids and sorbents production
    In the present article we introduce application areas of Сhlorella sorokiniana microalgae biomass for national economy (feed and biologically active additives, etc). Here it is shown that thermal and chemical modification of plant material waste might result in obtaining of highlyefficient materials for water purification. Major stages of waste-free technology of microalga processing with further extraction of valuable lipids and obtaining of sorption materials are schematically shown. We have determined fatty-acid content of lipid fraction (77 mg/g), obtained by Soxhlet method from lyophilized microalga biomass. It was revealed, that it includes 83.7% of unsaturated fatty acids (generally, С 18:1 , С 18:2 , С 18:3 ), whereas saturated fatty acids are mostly presented by С 16 - С 20 family. In lyophilized biomass we discovered α-linolenoic acid (Omega-3, 28.3%), which is one of the most valuable biologically active compounds, required for full-value human life and activities. Microstructure analysis of residual biomass of C. sorokiniana microalgae has shown pore surface, formed by destroyed cells. In the course of studying the sorption properties of residual biomass with the use of initial and final concentration parameters, the efficiency of purification of model water from Fe 3+ ions (47.5%) was evaluated, which indicates the need to modify the sorption properties of biomass. It has been established that the degree of wastewater purification containing iron (III) compounds can be significantly increased (by 1.8 times) by forming from the residual biomass granular materials with additional introduction of chitosan in the composition, which using as a binder and a modifying additive (mass ratio 4:1).
    Publicationtype: Journal Article
      95