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Item type:Publication, Access status: without files , Metallibacterium scheffleri gen. nov., sp. nov., an alkalinizing gammaproteobacterium isolated from an acidic biofilm(Microbiology Society, 2013-04-01); ; ; ; A Gram-stain-negative, non-motile, facultatively anaerobic, acid-tolerant rod, designated strain DKE6T, was isolated from an acidic biofilm (pH 2.5) harvested in the pyrite mine Drei Kronen und Ehrt in Germany. The isolate grew optimally at pH 5.5, between 25 and 30 °C and only with casein as the carbon and energy source; although a variety of sugars were tested as growth substrates, none supported growth of the isolate. During casein consumption, strain DKE6T produced and hence modulate the pH towards the growth optimum. The predominant fatty acids (>5%) were iso-C11:0 3-OH, iso-C15:0, iso-C17:0 and iso-C17:1 ω9c. The DNA G+C content was 66.6%. Strain DKE6T was not able to oxidize iron or thiosulfate. Iron reduction was detected. The isolate showed 93.3% 16S rRNA gene sequence similarity to the most closely related cultivable strain, Dokdonella koreensis DS-123T, but <93.2% sequence similarity with other type strains of closely related type species of the Gammaproteobacteria. On the basis of physiological and biochemical data, the isolate is considered to represent a novel species of a new genus in the class Gammaproteobacteria, for which we propose the name Metallibacterium scheffleri gen. nov., sp. nov. The type strain of the type species is DKE6T (=DSM 24874T=JCM 17596T).Publicationtype: Journal ArticleScopus© Citations 65 11 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Leucobacter chromiiresistens sp. nov., a chromate-resistant strain(Microbiology Society, 2011-04-01); ; ; ; A Gram-positive, irregular rod-shaped, non-motile, yellow-pigmented bacterium, strain JG 31T, was isolated in the course of identifying chromium-resistant soil bacteria. 16S rRNA gene sequence analysis of the isolated bacterium indicated its phylogenetic position within the genus Leucobacter. Binary 16S rRNA gene sequence alignments of the isolated bacterium with the 11 species of the genus recognized at the time of writing revealed sequence similarities of more than 97% with Leucobacter alluvii (GenBank accession no: AM072820; 99.4%), Leucobacter iarius (AM040493; 98.2%), Leucobacter aridicollis (AJ781047; 97.8%), Leucobacter komagatae (AB007419; 97.4%), Leucobacter chironomi (EU346911; 97.1%) and Leucobacter luti (AM072819; 97.1%). In contrast, DNA-DNA hybridization experiments showed similarity values below 28% for DNA samples from the most closely related type strains of L. alluvii, L. aridicollis and L. iarius. Protein analysis by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and automated RiboPrinting using the restriction enzyme PvuII differentiated strain JG 31T from all type strains of the genus Leucobacter. The dominant fatty acids of the novel isolate were anteiso-C15: 0, anteiso-C17: 0 and iso-C16: 0, while the quinone system consisted of menaquinones MK-11, MK-10, MK-9 and MK-8. In a B-type cross-linked peptidoglycan, the cell-wall amino acids were alanine, glycine, threonine, glutamic acid and 2,4-diaminobutyric acid. Strain JG 31T was able to grow in a medium containing up to 300 mM K2CrO4 and showed cellular aggregation in response to chromate stress. From biochemical and genomic analyses, the new strain is considered to represent a novel species of the genus Leucobacter, for which the name Leucobacter chromiiresistens sp. nov. is proposed. The type strain is strain JG 31T (=DSM 22788T =CCOS 200T).Publicationtype: Journal ArticleScopus© Citations 36 17