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Item type:Publication, Access status: with files , Model-based optimization of enzyme cocktails for sustainable lignocellulosic biomass saccharification(Elsevier, 2026-09-16); ; Lignocellulosic biomass has a high potential as resource for the chemical industry, but its conversion to fermentable sugars requires efficient pre-treatment and a subsequent enzymatic saccharification. Here, we present a model-based workflow for the design of enzyme cocktails for lignocellulosic biomass saccharification. Saccharification performance depends on several factors, such as substrate structure and pre-treatment severity, enzyme synergy, and inhibition effects. Because of this complexity, optimization by experimental trial-and-error is expensive and time-consuming, and enzyme mixtures optimized for one substrate often lack transferability to other feedstocks. To address these challenges, this work couples a stochastic simulator of lignocellulose hydrolysis with multi-objective optimization, explicitly resolving the trade-off between conversion speed and enzyme usage as a proxy for costs. The results demonstrate the feasibility and potential of model-based optimisation for rational enzyme cocktail design.Publicationtype: Journal Article5 2 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Bioreactive separation technology: a retrospective and perspective(Elsevier, 2026-03-28); ; ; ; Bluemke, WilfriedBiocatalysis, using enzymes or whole cells, offers high selectivity under mild conditions, but its broader application is often hindered by slow kinetics, equilibrium limitations, product inhibition and the processing of dilute streams in the presence of enzymes and cells. Bioreactive separation, defined as the simultaneous process of biocatalysis and separation, provides a powerful concept to overcome these barriers and therefore offers the potential of superiority over conventional processes. The current retro- and perspective paper, which is the result of an interdisciplinary workshop with academic and industry experts from the areas of biotechnology, fluid separations and process systems engineering, provides a focussed review, paired with a distinct novel definition of bioreactive separations that links to multifunctional reactors in chemical engineering and in situ product removal in biocatalysis. By establishing a common framework, this definition connects different research areas and enables systematic development. The current status of bioreactive separation processes is evaluated using SWOT analyses to identify key potentials and challenges. Based on this, a vision for 2040 is outlined, highlighting the need for advances in integration strategies, biocatalyst design, modelling and simulation, and applied research. Overall, a coordinated progress in these areas can significantly enhance the scalability and sustainability of bioprocessing.Publicationtype: Review Article48 31