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    Item type:Publication, Access status: with files ,
    Description of solutes/solids interactions by supercritical fluid chromatography
    (2023)
    Sun, Miaotian
    ;
    Gurikov, Pavel
    Abstract Knowing the interactions between a solute and an adsorbent is of great importance for the design of various green processes which utilize supercritical carbon dioxide (sc-CO2) as a solvent. In this work, by means of supercritical fluid chromatography (SFC) the thermodynamics of such systems have been studied. A wide range of polar and nonpolar solutes was used whereas methanol acted as the modifier. The retention data for various systems on four silica matrices was collected at various pressures, temperatures and modifier concentrations by SFC. Subsequently, two different types of retention models, one focusing on solute properties and the other modifier effects, were developed with the goal to generate a more accurate description of the solute-solid-interactions in sc-CO2.
    Publicationtype: Doctoral Thesis
      131  249
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    Item type:Publication, Access status: with files ,
    Novel strategies for automated engineering of enzymatic systems: structural and functional insights to human pyruvate dehydrogenase complex
    (2021-02-15)
    Ilhan, Sibel
    ;
    Zeng, An-Ping
    This thesis comprises the development of a process strategy for the targeted exploitation of beneficial enzyme properties via rapid and automated protein production and screening. Furthermore, to provide a better understanding of the structure and functionality of the human pyruvate dehydrogenase complex (hPDC). Structurally, interactions of different protein domains within hPDC were investigated using truncated core variants. Moreover, the hPDC activity was examined by multi-parameter screening, whereat an oscillating behaviour of hPDC was determined experimentally under certain parameter conditions. Finally, an automated process chain was developed to manipulate DNA sequences, produce proteins and subsequently test for functionality within few hours.
    Publicationtype: Doctoral Thesis
      323  1075