2 results
Now showing1 - 2 of 2
- Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Systematic analysis of jurkat T-cell deformation in fluorescence microscopy data(Springer, 2017-03); ; ; ; In the adaptive immune system, Calcium (Ca2+) is acting as a fundamental on-switch. Fluorescence microscopy is used to study the underlying mechanisms. However, living cells introduce motion and for the analysis of (sub-)cellular Ca2+ activity a precise motion analysis is necessary. We present an image based workflow to detect and analyze cell motion. We evaluate our approach on Jurkat T-cells using cell motion as observed from actual time series of cell images. Results indicate, that our method is able to detect deformation with an error of 0.2222 ± 0.086μm which is in the range of the image resolution, showing that accurate cell deformation detection is possible and feasible.Publicationtype: Conference Paper119 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , A modular framework for post-processing and analysis of fluorescence microscopy image sequences of subcellular calcium dynamics(Springer, 2015-03); ; ; ; Calcium (Ca2+) signaling is essential for activation of Tlymphocytes and can be understood as fundamental on-switch for the adaptive immune system. The activation is supposed to start by initial spatially and temporally localized Ca2+ signals. Imaging and analysis of these signals require high spatio-temporal resolution fluorescence microscopy – which, in turn, results in the need for an efficient and reliable post-processing and analysis workflow of the acquired image data. Started with a well established but time-consuming post-processing process, we report on our efforts to automatize and optimize it. The efforts led to a modular post-processing and analysis framework, which is presented. In addition, the influence of instances of the main blocks of the framework (e.g. bleaching correction, deconvolution) on Ca2+ dynamics analysis measures is evaluated.Publicationtype: Conference Paper86