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Optimization of solvent-free enzymatic esterification in eutectic substrate reaction mixture
Citation Link: https://doi.org/10.15480/882.2205
Publikationstyp
Journal Article
Publikationsdatum
2019
Sprache
English
Institut
TORE-URI
Enthalten in
Volume
24
Start Page
e00333
Citation
Biotechnology report (22): e00333 (2019)
Publisher DOI
Scopus ID
The Candida rugosa lipase catalyzed esterification of (-)-menthol and lauric acid (LA) was studied in a eutectic mixture formed by both substrates((-)-menthol:LA 3:1, mol/mol). No additional reaction solvent was necessary, since the (-)-menthol:LA deep eutectic solvent (DES) acts as combined reaction medium and substrate pool. Therefore, the esterification is conducted under solvent-free conditions. The thermodynamic water activity (aw) was identified as a key parameter affecting the esterification performance in the (-)-menthol:LA DES. A response surface methodology was applied to optimize the esterification conditions in terms aw, amount of C. rugosa lipase (mCRL) and reaction temperature. Under the optimized reaction conditions (aw = 0.55; mCRL =60 mg; T =45 °C), a conversion of 95 ± 1% LA was achieved (one day), the final (-)-menthyl lauric acid ester concentration reached 1.36 ± 0.04 M (2.25 days). The experimental product formation rate agreed very well with the model prediction.
Schlagworte
deep eutectic solvent
biocatalysis
water activity
reaction medium engineering
response surface methodology
design of experiments
DDC Class
500: Naturwissenschaften
More Funding Information
BMBF
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