TUHH Open Research
Help
  • Log In
    New user? Click here to register.Have you forgotten your password?
  • English
  • Deutsch
  • Communities & Collections
  • Publications
  • Research Data
  • People
  • Institutions
  • Projects
  • Statistics
  1. Home
  2. TUHH
  3. Publications
  4. Gene regulatory network analysis identifies MYL1, MDH2, GLS, and TRIM28 as the principal proteins in the response of mesenchymal stem cells to Mg²⁺ ions
 
Options

Gene regulatory network analysis identifies MYL1, MDH2, GLS, and TRIM28 as the principal proteins in the response of mesenchymal stem cells to Mg²⁺ ions

Citation Link: https://doi.org/10.15480/882.9562
Publikationstyp
Journal Article
Date Issued
2024-12-01
Sprache
English
Author(s)
Nourisa, Jalil
Passemiers, Antoine
Shakeri, Farhad
Omidi, Maryam
Helmholz, Heike  
Raimondi, Daniele
Moreau, Yves
Tomforde, Sven
Schlüter, Hartmuth
Luthringer-Feyerabend, Bérengère J. C.  
Cyron, Christian J.  
Kontinuums- und Werkstoffmechanik M-15  
Aydin, Roland 
Machine Learning in Virtual Materials Design M-EXK5  
Willumeit-Römer, Regine  
Zeller-Plumhoff, Berit  
TORE-DOI
10.15480/882.9562
TORE-URI
https://hdl.handle.net/11420/47426
Journal
Computational and structural biotechnology journal  
Volume
23
Start Page
1773
End Page
1785
Citation
Computational and Structural Biotechnology Journal 23: 1773–1785 (2024)
Publisher DOI
10.1016/j.csbj.2024.04.033
Scopus ID
2-s2.0-85190945469
Publisher
Elsevier
Magnesium (Mg)-based implants have emerged as a promising alternative for orthopedic applications, owing to their bioactive properties and biodegradability. As the implants degrade, Mg2+ ions are released, influencing all surrounding cell types, especially mesenchymal stem cells (MSCs). MSCs are vital for bone tissue regeneration, therefore, it is essential to understand their molecular response to Mg2+ ions in order to maximize the potential of Mg-based biomaterials. In this study, we conducted a gene regulatory network (GRN) analysis to examine the molecular responses of MSCs to Mg2+ ions. We used time-series proteomics data collected at 11 time points across a 21-day period for the GRN construction. We studied the impact of Mg2+ ions on the resulting networks and identified the key proteins and protein interactions affected by the application of Mg2+ ions. Our analysis highlights MYL1, MDH2, GLS, and TRIM28 as the primary targets of Mg2+ ions in the response of MSCs during 1–21 days phase. Our results also identify MDH2-MYL1, MDH2-RPS26, TRIM28-AK1, TRIM28-SOD2, and GLS-AK1 as the critical protein relationships affected by Mg2+ ions. By offering a comprehensive understanding of the regulatory role of Mg2+ ions on MSCs, our study contributes valuable insights into the molecular response of MSCs to Mg-based materials, thereby facilitating the development of innovative therapeutic strategies for orthopedic applications.
Subjects
Gene regulatory network analysis
Magnesium ions
Mesenchymal stem cells
Proteomics
DDC Class
610: Medicine, Health
Publication version
publishedVersion
Lizenz
https://creativecommons.org/licenses/by/4.0/
Loading...
Thumbnail Image
Name

1-s2.0-S2001037024001168-main.pdf

Type

Main Article

Size

4.53 MB

Format

Adobe PDF

TUHH
Weiterführende Links
  • Contact
  • Send Feedback
  • Cookie settings
  • Privacy policy
  • Impress
DSpace Software

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science
Design by effective webwork GmbH

  • Deutsche NationalbibliothekDeutsche Nationalbibliothek
  • ORCiD Member OrganizationORCiD Member Organization
  • DataCiteDataCite
  • Re3DataRe3Data
  • OpenDOAROpenDOAR
  • OpenAireOpenAire
  • BASE Bielefeld Academic Search EngineBASE Bielefeld Academic Search Engine
Feedback