Publisher DOI: 10.1128/AEM.02208-18
Title: Chromate resistance mechanisms in Leucobacter chromiiresistens
Language: English
Authors: Sturm, Gunnar 
Brunner, Stefanie 
Suvorova, Elena 
Dempwolff, Felix 
Reiner, Johannes Eberhard 
Graumann, Peter 
Bernier-Latmani, Rizlan 
Majzlan, Juraj 
Gescher, Johannes 
Keywords: Chromate resistance;Heavy metals;Heavy-metal resistance;Leucobacter
Issue Date: 1-Dec-2018
Source: Applied and Environmental Microbiology 84 (23): e02208-18 (2018-12-01)
Journal: Applied and environmental microbiology 
Abstract (english): 
Chromate is one of the major anthropogenic contaminants on Earth. Leucobacter chromiiresistens is a highly chromate-resistant strain, tolerating chromate concentrations in LB medium of up to 400 mM. In response to chromate stress, L. chromiiresistens forms biofilms, which are held together via extracellular DNA. Inhibition of biofilm formation leads to drastically decreased chromate tolerance. Moreover, chromate is reduced intracellularly to the less-toxic Cr(III). The oxidation status and localization of chromium in cell aggregates were analyzed by energy-dispersive X-ray spectroscopy coupled to scanning transmission electron microscopy and X-ray absorption spectroscopy measurements. Most of the heavy metal is localized as Cr(III) at the cytoplasmic membrane. As a new cellular response to chromate stress, we observed an increased production of the carotenoid lutein. Carotenoid production could increase membrane stability and reduce the concentration of reactive oxygen species. Bioinformatic analysis of the L. chromiiresistens genome revealed several gene clusters that could enable heavy-metal resistance. The extreme chromate tolerance and the unique set of resistance factors suggest the use of L. chromiiresistens as a new model organism to study microbial chromate resistance.
ISSN: 0099-2240
Document Type: Article
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