Dieses Dokument steht unter einer CreativeCommons Lizenz by/3.0
DC ElementWertSprache
dc.contributor.authorWeiss, Katja-
dc.contributor.authorSalzig, Denise-
dc.contributor.authorRöder, Yasmin-
dc.contributor.authorGerstenberger, Jarrid-
dc.contributor.authorMühlebach, Michael D.-
dc.contributor.authorCichutek, Klaus-
dc.contributor.authorPörtner, Ralf-
dc.contributor.authorCzermak, Peter-
dc.date.accessioned2019-02-01T09:19:35Z-
dc.date.available2019-02-01T09:19:35Z-
dc.date.issued2013-
dc.identifier.citationAmerican Journal of Biochemistry and Biotechnology 3 (9): 243-254 (2013)de_DE
dc.identifier.issn1558-6332de_DE
dc.identifier.urihttps://tubdok.tub.tuhh.de/handle/11420/2011-
dc.description.abstractRecombinant measles viruses are currently tested in clinical trials as oncolytic agent to be applied in cancer therapy. Contrary to their use as vaccine where 103infectious virus particles per dose are needed, for cancer therapy 109virus particles should be provided per dose. This leads to other challenges for the production process when compared to vaccine production. This study presents measles virus stability with regard to conditions during production and storage of the virus. Relevant process parameters such as temperature (4-37°C), pH (pH 4-11), conductivity (1.5 to 137.5 mS cm-1) and oxygen partial pressure were analyzed. The infectivity of measles virus particles decreased highly at 37 and 32°C, while at 22 and 4°C it remained stable for several hours or even days, respectively. The thermal inactivation reactions followed first order kinetics and the thermodynamic parameters enthalpy and entropy were estimated. Towards changes in pH measles virus particles were very sensitive, while no inactivation could be observed with varying conductivity. Measles virus incubation at an oxygen partial pressure of 100% did not lead to any loss of infectivity. The results show which parameters should be considered and controlled strongly in the production process to further raise measles virus yields for the high amount needed in cancer therapy approaches. © 2013 Science Publication.en
dc.description.sponsorshipDeutsche Krebshilfe; Bundesministerium für Bildung und Forschung; Hessisches Ministerium für Wissenschaft und Kunst (LOEWE-Programm)de_DE
dc.language.isoende_DE
dc.publisherScience Publicationsde_DE
dc.relation.ispartofAmerican journal of biochemistry and biotechnologyde_DE
dc.rightsCC BY 3.0de_DE
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.subjectMeasles Virus (MV)de_DE
dc.subjectVirus Stabilityde_DE
dc.subjectVirus Inactivationde_DE
dc.subjectOncolytic Agentde_DE
dc.subject.ddc570: Biowissenschaften, Biologiede_DE
dc.titleInfluence of process conditions on measles virus stabilityde_DE
dc.typeArticlede_DE
dc.identifier.urnurn:nbn:de:gbv:830-882.026347-
dc.identifier.doi10.15480/882.2008-
dc.type.diniarticle-
dc.subject.ddccode570-
dcterms.DCMITypeText-
tuhh.identifier.urnurn:nbn:de:gbv:830-882.026347-
tuhh.oai.showtruede_DE
dc.identifier.hdl11420/2011-
tuhh.abstract.englishRecombinant measles viruses are currently tested in clinical trials as oncolytic agent to be applied in cancer therapy. Contrary to their use as vaccine where 103infectious virus particles per dose are needed, for cancer therapy 109virus particles should be provided per dose. This leads to other challenges for the production process when compared to vaccine production. This study presents measles virus stability with regard to conditions during production and storage of the virus. Relevant process parameters such as temperature (4-37°C), pH (pH 4-11), conductivity (1.5 to 137.5 mS cm-1) and oxygen partial pressure were analyzed. The infectivity of measles virus particles decreased highly at 37 and 32°C, while at 22 and 4°C it remained stable for several hours or even days, respectively. The thermal inactivation reactions followed first order kinetics and the thermodynamic parameters enthalpy and entropy were estimated. Towards changes in pH measles virus particles were very sensitive, while no inactivation could be observed with varying conductivity. Measles virus incubation at an oxygen partial pressure of 100% did not lead to any loss of infectivity. The results show which parameters should be considered and controlled strongly in the production process to further raise measles virus yields for the high amount needed in cancer therapy approaches. © 2013 Science Publication.de_DE
tuhh.publisher.doi10.3844/ajbbsp.2013.243.254-
tuhh.publication.instituteBioprozess- und Biosystemtechnik V-1de_DE
tuhh.identifier.doi10.15480/882.2008-
tuhh.type.opus(wissenschaftlicher) Artikelde
tuhh.institute.germanBioprozess- und Biosystemtechnik V-1de
tuhh.institute.englishBioprozess- und Biosystemtechnik V-1de_DE
tuhh.gvk.hasppnfalse-
openaire.rightsinfo:eu-repo/semantics/openAccessde_DE
dc.type.driverarticle-
dc.rights.ccbyde_DE
dc.rights.ccversion3.0de_DE
dc.type.casraiJournal Articleen
tuhh.container.issue3de_DE
tuhh.container.volume9de_DE
tuhh.container.startpage243de_DE
tuhh.container.endpage254de_DE
dc.rights.nationallicensefalsede_DE
item.fulltextWith Fulltext-
item.creatorGNDWeiss, Katja-
item.creatorGNDSalzig, Denise-
item.creatorGNDRöder, Yasmin-
item.creatorGNDGerstenberger, Jarrid-
item.creatorGNDMühlebach, Michael D.-
item.creatorGNDCichutek, Klaus-
item.creatorGNDPörtner, Ralf-
item.creatorGNDCzermak, Peter-
item.creatorOrcidWeiss, Katja-
item.creatorOrcidSalzig, Denise-
item.creatorOrcidRöder, Yasmin-
item.creatorOrcidGerstenberger, Jarrid-
item.creatorOrcidMühlebach, Michael D.-
item.creatorOrcidCichutek, Klaus-
item.creatorOrcidPörtner, Ralf-
item.creatorOrcidCzermak, Peter-
item.grantfulltextopen-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.orcid0000-0001-9266-1356-
crisitem.author.orcid0000-0002-7069-5018-
crisitem.author.orcid0000-0003-1163-9718-
crisitem.author.orcid0000-0001-7485-500X-
Enthalten in den Sammlungen:Publications (tub.dok)
Dateien zu dieser Ressource:
Datei Beschreibung GrößeFormat
ajbbsp.2013.243.254.pdfVerlags-PDF322,73 kBAdobe PDFÖffnen/Anzeigen
Zur Kurzanzeige

Seitenansichten

41
Letzte Woche
0
Letzten Monat
checked on 26.03.2019

Download(s)

14
checked on 26.03.2019

Google ScholarTM

Prüfe

Export

Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons