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Publisher DOI: 10.1186/1471-2164-14-430
Title: Genetic variability of mutans streptococci revealed by wide whole-genome sequencing
Language: English
Authors: Song, Lifu 
Wang, Wei 
Conrads, Georg 
Rheinberg, Anke 
Sztajer, Helena 
Reck, Michael 
Wagner-Döbler, Irene 
Zeng, An-Ping  
Keywords: Mutans Streptococci;Streptococcus Mutans;Streptococcus Ratti;Streptococcus Sobrinus;Comparative Genomics,;Core-genome;Pan-genome;Pathogenicity;Lactate Oxidase;Metabolic Network
Issue Date: 28-Jun-2013
Publisher: BioMed Central ; Springer
Source: BMC Genomics 1 (14): 430 (2013)
Journal or Series Name: BMC genomics 
Abstract (english): Background: Mutans streptococci are a group of bacteria significantly contributing to tooth decay. Their genetic variability is however still not well understood.Results: Genomes of 6 clinical S. mutans isolates of different origins, one isolate of S. sobrinus (DSM 20742) and one isolate of S. ratti (DSM 20564) were sequenced and comparatively analyzed. Genome alignment revealed a mosaic-like structure of genome arrangement. Genes related to pathogenicity are found to have high variations among the strains, whereas genes for oxidative stress resistance are well conserved, indicating the importance of this trait in the dental biofilm community. Analysis of genome-scale metabolic networks revealed significant differences in 42 pathways. A striking dissimilarity is the unique presence of two lactate oxidases in S. sobrinus DSM 20742, probably indicating an unusual capability of this strain in producing H2O2 and expanding its ecological niche. In addition, lactate oxidases may form with other enzymes a novel energetic pathway in S. sobrinus DSM 20742 that can remedy its deficiency in citrate utilization pathway.Using 67 S. mutans genomes currently available including the strains sequenced in this study, we estimates the theoretical core genome size of S. mutans, and performed modeling of S. mutans pan-genome by applying different fitting models. An "open" pan-genome was inferred.Conclusions: The comparative genome analyses revealed diversities in the mutans streptococci group, especially with respect to the virulence related genes and metabolic pathways. The results are helpful for better understanding the evolution and adaptive mechanisms of these oral pathogen microorganisms and for combating them. © 2013 Song et al.; licensee BioMed Central Ltd.
DOI: 10.15480/882.2012
ISSN: 1471-2164
Institute: Bioprozess- und Biosystemtechnik V-1 
Type: (wissenschaftlicher) Artikel
Funded by: Bundesministerium für Bildung und Forschung
Project: Research Program “Medical systems biology - MedSys”, project “Development of biofilm inhibitors using a systems biology approach” (0315411) 
e:Bio-Modul II- Verbundprojekt: e:biofilm-Neue Wirkstoffe gegen Biofilme von Streptokokken-Teilprojekt B 
License: CC BY 2.0 (Attribution) CC BY 2.0 (Attribution)
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