DC FieldValueLanguage
dc.contributor.authorBesztejan, Stephanie-
dc.contributor.authorKeskin, Sercan-
dc.contributor.authorManz, Stephanie-
dc.contributor.authorKassier, Günther-
dc.contributor.authorBücker, Robert-
dc.contributor.authorVenegas-Rojas, Daybith-
dc.contributor.authorTrieu, Hoc Khiem-
dc.contributor.authorRentmeister, Andrea-
dc.contributor.authorMiller, R. J. Dwayne-
dc.date.accessioned2019-11-07T12:51:23Z-
dc.date.available2019-11-07T12:51:23Z-
dc.date.issued2017-01-31-
dc.identifier.citationMicroscopy and Microanalysis 1 (23): 46-55 (2017-02-01)de_DE
dc.identifier.issn1435-8115de_DE
dc.identifier.urihttp://hdl.handle.net/11420/3752-
dc.description.abstractWe present liquid-cell transmission electron microscopy (liquid-cell TEM) imaging of fixed and non-fixed prostate cancer cells (PC3 and LNCaP) with high resolution in a custom developed silicon nitride liquid cell. Fixed PC3 cells were imaged for 90–120 min without any discernable damage. High contrast on the cellular structures was obtained even at low electron doses (~2.5 e − /nm 2 per image). The images show distinct structures of cell compartments (nuclei and nucleoli) and cell boundaries without any further sample embedding, dehydration, or staining. Furthermore, we observed dynamics of vesicles trafficking from the cell membrane in consecutive still frames in a non-fixed cell. Our findings show that liquid-cell TEM, operated at low electron dose, is an excellent tool to investigate dynamic events in non-fixed cells with enough spatial resolution (few nm) and natural amplitude contrast to follow key intracellular processes.en
dc.description.sponsorshipThis work was funded by the Max Planck Society and supported by the excellence cluster “The Hamburg Centre for Ultrafast Imaging”.de_DE
dc.language.isoende_DE
dc.publisherCambridge University Pressde_DE
dc.relation.ispartofMicroscopy and microanalysisde_DE
dc.subjectGold nanoparticlesde_DE
dc.subjectIn situ imagingde_DE
dc.subjectLiquid-cell TEMde_DE
dc.subjectMammalian cellsde_DE
dc.subjectNanofluidic cellde_DE
dc.subject.ddc600: Technikde_DE
dc.titleVisualization of cellular components in a Mammalian cell with liquid-cell transmission electron microscopyde_DE
dc.typeArticlede_DE
dc.type.diniarticle-
dc.subject.ddccode600-
dcterms.DCMITypeText-
tuhh.abstract.englishWe present liquid-cell transmission electron microscopy (liquid-cell TEM) imaging of fixed and non-fixed prostate cancer cells (PC3 and LNCaP) with high resolution in a custom developed silicon nitride liquid cell. Fixed PC3 cells were imaged for 90–120 min without any discernable damage. High contrast on the cellular structures was obtained even at low electron doses (~2.5 e − /nm 2 per image). The images show distinct structures of cell compartments (nuclei and nucleoli) and cell boundaries without any further sample embedding, dehydration, or staining. Furthermore, we observed dynamics of vesicles trafficking from the cell membrane in consecutive still frames in a non-fixed cell. Our findings show that liquid-cell TEM, operated at low electron dose, is an excellent tool to investigate dynamic events in non-fixed cells with enough spatial resolution (few nm) and natural amplitude contrast to follow key intracellular processes.de_DE
tuhh.publisher.doi10.1017/S1431927616012708-
tuhh.publication.instituteMikrosystemtechnik E-7de_DE
tuhh.type.opus(wissenschaftlicher) Artikel-
tuhh.institute.germanMikrosystemtechnik E-7de
tuhh.institute.englishMikrosystemtechnik E-7de_DE
tuhh.gvk.hasppnfalse-
dc.type.driverarticle-
dc.type.casraiJournal Article-
tuhh.container.issue1de_DE
tuhh.container.volume23de_DE
tuhh.container.startpage46de_DE
tuhh.container.endpage55de_DE
dc.relation.projectNanofluidikchip für in-liquid TEM-
local.status.inpressfalsede_DE
item.creatorGNDBesztejan, Stephanie-
item.creatorGNDKeskin, Sercan-
item.creatorGNDManz, Stephanie-
item.creatorGNDKassier, Günther-
item.creatorGNDBücker, Robert-
item.creatorGNDVenegas-Rojas, Daybith-
item.creatorGNDTrieu, Hoc Khiem-
item.creatorGNDRentmeister, Andrea-
item.creatorGNDMiller, R. J. Dwayne-
item.languageiso639-1en-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.grantfulltextnone-
item.creatorOrcidBesztejan, Stephanie-
item.creatorOrcidKeskin, Sercan-
item.creatorOrcidManz, Stephanie-
item.creatorOrcidKassier, Günther-
item.creatorOrcidBücker, Robert-
item.creatorOrcidVenegas-Rojas, Daybith-
item.creatorOrcidTrieu, Hoc Khiem-
item.creatorOrcidRentmeister, Andrea-
item.creatorOrcidMiller, R. J. Dwayne-
crisitem.author.deptMikrosystemtechnik E-7-
crisitem.author.deptMikrosystemtechnik E-7-
crisitem.author.orcid0000-0001-7382-1799-
crisitem.author.orcid0000-0001-7654-4707-
crisitem.author.orcid0000-0002-3107-4147-
crisitem.author.orcid0000-0003-0884-0541-
crisitem.author.parentorgStudiendekanat Elektrotechnik, Informatik und Mathematik-
crisitem.author.parentorgStudiendekanat Elektrotechnik, Informatik und Mathematik-
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